MYL3/CMLC1 anticorps (C-Term)

N° du produit ABIN7877434

Cet anticorps anti-MYL3/CMLC1 Polyclonal Lapin (ABIN7877434) détecte spécifiquement MYL3/CMLC1 dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MYL3/CMLC1 anticorps (C-Term) (ABIN7877434)

Antigène: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYL3/CMLC1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-MYL3/CMLC1 anticorps

Épitope: C-Term

Fonction: MYL3 Antibody / Myosin light chain 3

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human MYL3 was used as the immunogen for the MYL3 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the MYL3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the MYL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur MYL3/CMLC1

Antigène: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Autre désignation: MYL3

Sujet: MYL3 antibody detects Myosin light chain 3, a contractile protein component of cardiac and skeletal muscle that modulates actin-myosin interactions during muscle contraction. The UniProt recommended name is Myosin light chain 3 (MYL3). This protein binds to myosin heavy chains and contributes to force generation and sarcomere stability in striated muscle fibers.

Functionally, MYL3 antibody identifies a 151-amino-acid calcium-binding protein localized to the myofilament's thick filament region. MYL3 interacts with the myosin heavy chain alpha isoform and regulates actomyosin ATPase activity through conformational changes induced by calcium binding. It plays a key role in sarcomere assembly, contractile force regulation, and muscle fiber maintenance.

The MYL3 gene is located on chromosome 3p21.31 and is expressed predominantly in ventricular cardiac tissue and slow-twitch skeletal muscles. MYL3 is essential for normal heart contractility and mechanical stability under physiological stress.

Pathologically, mutations in MYL3 cause familial hypertrophic cardiomyopathy and restrictive cardiomyopathy by altering calcium binding and myosin-actin interactions. Defects in MYL3 impair contractile performance and promote myofibrillar disarray. Research using MYL3 antibody supports studies in cardiac physiology, muscle biochemistry, and hereditary myopathies.

MYL3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sarcomeric proteins. NSJ Bioreagents provides MYL3 antibody reagents optimized for research in cardiomyocyte biology, muscle contraction, and structural myopathy studies.

Structurally, Myosin light chain 3 belongs to the EF-hand superfamily and contains two calcium-binding motifs that regulate its conformational changes and interaction with myosin. This antibody aids analysis of MYL3's role in cardiac contractility and muscle development.

UniProt: P08590