LIG4 anticorps (AA 1-876) (Biotin)

N° du produit ABIN8005986

L’anticorps anti-LIG4 Polyclonal Lapin est utilisé pour la détection de LIG4 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC et ELISA.

Aperçu rapide pour LIG4 anticorps (AA 1-876) (Biotin) (ABIN8005986)

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG4 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-LIG4 anticorps (Biotin)

Épitope: AA 1-876

Fonction: Anti-DNA Ligase IV/LIG4 Antibody Biotin Conjugated

Réactivité croisée (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876).

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur LIG4

Antigène: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Autre désignation: LIG4

Sujet:

Background: DNA ligase 4 is an enzyme that in humans is encoded by the LIG4 gene. The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene Full Name: DNA ligase 4

ID gène: 3981

UniProt: P49917

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response