POLB anticorps (AA 122-335) (APC)

N° du produit ABIN8024094

L’anticorps anti-POLB Polyclonal Lapin est utilisé pour la détection de POLB dans des échantillons de Humain, Souris et Rat. Il a été validé pour FACS.

Aperçu rapide pour POLB anticorps (AA 122-335) (APC) (ABIN8024094)

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLB est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Détail du produit anti-POLB anticorps (APC)

Épitope: AA 122-335

Fonction: Anti-DNA Polymerase beta/POLB Antibody APC Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: Human POLB shares 95.3%,94.9% amino acid (aa) sequence identity with mouse,rat POLB,respectively.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Date de péremption: 12 months

Détails sur POLB

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Autre désignation: POLB

Sujet:

Background: Polymerase (DNA ed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

Gene Full Name: DNA polymerase beta

ID gène: 5423

UniProt: P06746

Pathways: Réparation de l'ADN