SPHK1 anticorps (AA 16-267)

N° du produit ABIN8041706

Cet anticorps Lapin Polyclonal détecte spécifiquement SPHK1 dans WB, ELISA, IHC, FACS, IF et ICC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour SPHK1 anticorps (AA 16-267) (ABIN8041706)

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SPHK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Classe de qualité: Carrier-free

Détail du produit anti-SPHK1 anticorps

Épitope: AA 16-267

Fonction: Anti-SPHK1 Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SPHK1 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SPHK1 recombinant protein (Position: R16-H267).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur SPHK1

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Autre désignation: SPHK1

Sujet:

Background: SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Gene Full Name: sphingosine kinase 1

Poids moléculaire: 45-50 kDa

ID gène: 8877

UniProt: Q9NYA1

Pathways: VEGF Signaling