RAD9A anticorps (pSer272)

N° du produit ABIN8124223

Cet anticorps anti-RAD9A Polyclonal Lapin (ABIN8124223) détecte spécifiquement RAD9A dans WB et ELISA. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour RAD9A anticorps (pSer272) (ABIN8124223)

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD9A est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RAD9A anticorps

Épitope: pSer272

Fonction: RAD9 (Phospho Ser272) Antibody

Specificité: This antibody detects endogenous levels of Human RAD9 (Phospho Ser272)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogène: Synthesized peptide derived from human RAD9 (Phospho Ser272)

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur RAD9A

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Autre désignation: RAD9

Sujet:

Background: catalytic activity:Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.,function:Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.,PTM:Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.,similarity:Belongs to the rad9 family.,subunit:Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2.,

Gene Name: RAD9

Protein Name: RAD9 (Phospho Ser272)

UniProt: Q96C41

Pathways: Positive Regulation of Response to DNA Damage Stimulus