NRF2 anticorps (AA 445-458)

N° du produit ABIN768601

Détail du produit anti-NRF2 anticorps

Antigène: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Épitope: AA 445-458

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NRF2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: NRF2 (aa445-458)

Séquence: KHSSRLEAHL TRDE

Specificité: This antibody is expected to recognize all reported isoforms (

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-KHSSRLEAHLTRDE, from the internal region of the protein sequence according to NP_006155.2, NP_001138884.1, NP_001138885.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 70 kDa band observed in Human Lung lysates and approx 75 kDa in preliminary testing of Mouse and Rat Liver and KNRK, MDCK, and nuclear NIH3T3 cell lysate (calculated MW of 67.8 kDa according to NP_006155.2). This molecular weight rate

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur NRF2

Antigène: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Autre désignation: NFE2L2 (NFE2L2 Produits)

Sujet: NFE2L2, nuclear factor (erythroid-derived 2)-like 2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2, OTTHUMP00000205251, OTTHUMP00000207980, nuclear factor erythroid 2-related factor 2, nuclear factor, erythroid derived 2, like 2

Poids moléculaire: 67.8kDa according to NP_006155.2

ID gène: 4780, 18024, 83619

NCBI Accession: NP_006155, NP_001138884, NP_001138885

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling