PDGFRA anticorps (AA 1035-1053)

N° du produit ABIN790499

Il existe 1 publication pour ce produit. L’anticorps anti-PDGFRA Polyclonal Lapin est utilisé pour la détection de PDGFRA dans des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Singe, Porc, Lapin, Cheval, Xenopus laevis, Roussette (Chauve-souris) et Hamster. Il a été validé pour WB, IHC, IHC (p) et IP.

Aperçu rapide pour PDGFRA anticorps (AA 1035-1053) (ABIN790499)

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Singe, Porc, Lapin, Cheval, Xenopus laevis, Roussette (Chauve-souris), Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDGFRA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-PDGFRA anticorps

Épitope: AA 1035-1053

Specificité: Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C

Stockage commentaire: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-PDGFRA (ABIN790499)

Nance, Shi, Hakim, Wasala, Yue, Pan, Zhang, Robinson, Duan, Yao, Yang, Chen, Wagner, Gersbach, Duan: "AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice." dans: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 9, pp. 1568-1585, (2019) (PubMed).

Détails sur PDGFRA

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Autre désignation: PDGFRA / PDGFR Alpha

Sujet: Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

ID gène: 5156

UniProt: P16234

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling