PDGFRA anticorps (AA 1035-1053)

N° du produit ABIN790499

L’anticorps Lapin Polyclonal anti-PDGFRA a été validé pour WB, IHC, IHC (p) et IP. Il convient pour détecter PDGFRA dans des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Singe, Porc, Lapin, Cheval, Xenopus laevis, Roussette (Chauve-souris) et Hamster. Il y a 1 publication disponible.

Aperçu rapide pour PDGFRA anticorps (AA 1035-1053) (ABIN790499)

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Singe, Porc, Lapin, Cheval, Xenopus laevis, Roussette (Chauve-souris), Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDGFRA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-PDGFRA anticorps

Épitope: AA 1035-1053

Marque: IHC-plus™

Specificité: Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C

Stockage commentaire: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-PDGFRA (ABIN790499)

Nance, Shi, Hakim, Wasala, Yue, Pan, Zhang, Robinson, Duan, Yao, Yang, Chen, Wagner, Gersbach, Duan: "AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice." dans: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 9, pp. 1568-1585, (2019) (PubMed).

Détails sur PDGFRA

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Autre désignation: PDGFRA / PDGFR Alpha

Sujet: Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

ID gène: 5156

UniProt: P16234

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling