Souris anti-Humain IgM (Fc Region) Anticorps (FITC)

Détails pour le produit réf. ABIN94403, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Fc Region
503
377
368
59
57
56
53
20
5
5
2
1
1
1
1
Reactivité
Humain
1191
592
242
97
85
76
75
64
60
59
59
43
39
28
19
19
19
11
5
5
2
1
1
1
Hôte
Souris
1277
678
590
142
41
16
10
4
3
3
3
1
Clonalité (Clone)
Monoclonal ()
Conjugué
FITC
365
352
284
136
97
79
67
52
42
24
24
22
21
20
20
20
18
16
16
15
13
12
9
8
8
8
8
8
8
8
8
8
8
7
6
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
Options
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N° du produit (Fournisseur)
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Immunogène Purified human IgM.
Clone CH2
Isotype IgG
Specificité The antibody CH2 reacts with Fc fragment of human IgM.
Attributs du produit The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Purification Purified from rabbit serum by protein-G affinity chromatography.
Sujet Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Poids moléculaire 900 kDa
Indications d'application The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Commentaires

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Images (Fournisseur)
Flow Cytometry (FACS) image for Mouse anti-Human IgM (Fc Region) antibody (FITC) (ABIN94403) Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." dans: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." dans: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." dans: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." dans: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).