STAT1 anticorps

N° du produit ABIN94471

Cet anticorps Souris Monoclonal détecte spécifiquement STAT1 dans WB, IP et IHC (p). Il présente une réactivité envers Humain et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour STAT1 anticorps (ABIN94471)

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp STAT1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SM1

Détail du produit anti-STAT1 anticorps

Fonction: Anti-STAT1 Purified

Specificité: The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

Réactivité croisée (Details): Mouse, Human, Other not tested

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: lung macrophages.
Western blotting: Recommended dilution 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-STAT1 (ABIN94471)

Kovarik, Boudny, Kocak, Lauerova, Fait, Vagundova: "Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation." dans: International journal of molecular medicine, Vol. 12, Issue 3, pp. 335-40, (2003) (PubMed).

Boudný, Kocák, Lauerová, Kovarík: "Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients." dans: Folia biologica, Vol. 49, Issue 4, pp. 142-6, (2003) (PubMed).

Détails sur STAT1

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Autre désignation: STAT1

Sujet: Signal transducer and activator of transcription 1,STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.,CANDF7, IMD31, ISGF3

ID gène: 6772

UniProt: P42224

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events