Nestin anticorps

N° du produit ABIN953652

Cet anticorps anti-Nestin est un anticorps Poulet Polyclonal détectant Nestin dans WB, IF et IHC (p). Adapté pour Souris et Rat.

Aperçu rapide pour Nestin anticorps (ABIN953652)

Antigène: Nestin (NES)

Reactivité: Souris, Rat

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Nestin anticorps

Specificité: Recognizes Nestin. Note that the sequences of the Mouse and Human Nestin genes are divergent, and it is unlikely that antibodies raised against the Mouse sequence would recognize Human Nestin.

Purification: Affinity Chromatography using a peptide column.

Immunogène: Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.3 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted in the dark at 2-8 °C.

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin

Sujet: Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.Synonyms: NES, Nbla00170

ID gène: 18008

NCBI Accession: NP_057910