OspC anticorps

N° du produit ABIN964717

Détail du produit

Antigène: OspC

Reactivité: Borrelia burgdorferi

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB)

Specificité: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.

Attributs du produit: Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.

Purification: purified

Stérilité: Sterile filtered

Immunogène: MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
Immunogen Type: RecombinantProtein

Isotype: IgG

Information d'application

Indications d'application: This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: ospC, BB_B19

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Référence

Karvonen, Tammisto, Nykky, Gilbert: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." dans: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).

Joung, Ballard, Ma, Tseng, Teshome, Burakowski, Garner, Di Carlo, Ozcan: "Paper-based multiplexed vertical flow assay for point-of-care testing." dans: Lab on a chip, Vol. 19, Issue 6, pp. 1027-1034, (2019) (PubMed).

Caine, Lin, Kessler, Sato, Leong, Coburn: "Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival." dans: Cellular microbiology, Vol. 19, Issue 12, (2018) (PubMed).

Caine, Coburn: "A short-term Borrelia burgdorferi infection model identifies tissue tropisms and bloodstream survival conferred by adhesion proteins." dans: Infection and immunity, Vol. 83, Issue 8, pp. 3184-94, (2015) (PubMed).

Détail du antigène

Antigène: OspC

Sujet: Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.
Synonyms: OspC, Borrelia burgdorferi OspC, PC, Outer Surface Protein C

ID gène: 1194415

NCBI Accession: NP_047005

UniProt: Q07337