IGF1R anticorps

N° du produit ABIN966348

Cité dans 2 publications. Cet anticorps anti-IGF1R Monoclonal (ABIN966348) détecte spécifiquement IGF1R dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour IGF1R anticorps (ABIN966348)

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivité: Humain

Hôte: Veuillez nous consulter SVP

Clonalité: Monoclonal

Conjugué: Cet anticorp IGF1R est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-IGF1R anticorps

Purification: Crude ascites.

Immunogène: Ni-NTA purified truncated recombinant IGF1R-His expressed in E. Coli strain BL21 (DE3)

Isotype: IgG2b

Information d'application

Indications d'application: Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
IHC(F): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: -20 °C

Références pour anticorps anti-IGF1R (ABIN966348)

Scheidegger, Cenni, Picard, Delafontaine: "Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects." dans: The Journal of biological chemistry, Vol. 275, Issue 49, pp. 38921-8, (2001) (PubMed).

Fujimoto, Tomita, Wakamatsu, Tanimoto, Ike: "Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response." dans: Journal of bacteriology, Vol. 177, Issue 19, pp. 5574-81, (1995) (PubMed).

Détails sur IGF1R

Antigène: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Autre désignation: IGF1R

Sujet: IGF1R(insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Pathways: Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy