ASK1 anticorps

N° du produit ABIN966515

Il existe 3+ publications pour ce produit. L’anticorps anti-ASK1 Monoclonal Souris est utilisé pour la détection de ASK1 dans des échantillons de Humain. Il a été validé pour WB, IHC, ELISA, IF et FACS.

Aperçu rapide pour ASK1 anticorps (ABIN966515)

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ASK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2E4

Détail du produit anti-ASK1 anticorps

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of human MAP3K5 expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-ASK1 (ABIN966515)

Pressinotti, Klocker, Schäfer, Luu, Ruschhaupt, Kuner, Steiner, Poustka, Bartsch, Sültmann: "Differential expression of apoptotic genes PDIA3 and MAP3K5 distinguishes between low- and high-risk prostate cancer." dans: Molecular cancer, Vol. 8, pp. 130, (2010) (PubMed).

Widenmaier, Ao, Kim, Warnock, McIntosh et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent ..." dans: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, (2009) (PubMed).

Woolcott, SenGupta, Hanna, Aronson: "Estrogen and progesterone receptor levels in nonneoplastic breast epithelium of breast cancer cases versus benign breast biopsy controls." dans: BMC cancer, Vol. 8, pp. 130, (2008) (PubMed).

Détails sur ASK1

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Autre désignation: MAP3K5

Sujet: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun Nterminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Synonyms: ASK1, MEKK5, MAPKKK5

Poids moléculaire: 55kDa

ID gène: 4217

Pathways: Signalisation MAPK, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response