CD247 anticorps (C-Term)

N° du produit ABIN967446

Cet anticorps anti-CD247 est un anticorps Souris Monoclonal détectant CD247 dans WB et IP. Adapté pour Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CD247 anticorps (C-Term) (ABIN967446)

Antigène: CD247 (CD247 Molecule (CD247))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD247 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 1zeta3A1

Détail du produit anti-CD247 anticorps

Épitope: C-Term

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse CD3zeta C-terminus synthetic peptide

Isotype: IgG2b

Information d'application

Indications d'application: Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10 [6] cells). Immunoprecipitation application is not routinely tested, only tested during development.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD247 (ABIN967446)

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, (1993) (PubMed).

Détails sur CD247

Antigène: CD247 (CD247 Molecule (CD247))

Autre désignation: CD247

Sujet: The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: gamma, delta, epsilon, zeta, and eta. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1zeta3A1 recognizes an epitope in the C-terminus of mouse CD3zeta. A synthetic peptide corresponding to the C-terminus of mouse CD3zeta (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.
Synonyms: CD3zeta chain

Poids moléculaire: 32 kDa

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway