SRSF2 anticorps

N° du produit ABIN967516

Détail du produit anti-SRSF2 anticorps

Antigène: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SRSF2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Partially purified spliceosomes

Clone: AlphaSC35

Isotype: IgG1 kappa

Information d'application

Indications d'application: Staining is nuclear, a punctate or speckled nuclear pattern may be detected. Only a subset of cells are stained, the percentage may vary between cell type and experimental conditions. MG-63 human osteosarcoma, HeLa carcinoma (ATCC CCL-2), or BRL-3A rat liver cells (ATCC CRL-1442) are suggested as positive controls.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-SRSF2 (ABIN967516)

Sukegawa, Blobel: "A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor." dans: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15702-6, (1995) (PubMed).

Fu: "Specific commitment of different pre-mRNAs to splicing by single SR proteins." dans: Nature, Vol. 365, Issue 6441, pp. 82-5, (1993) (PubMed).

Fu, Maniatis: "Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus." dans: Nature, Vol. 343, Issue 6257, pp. 437-41, (1990) (PubMed).

Détails sur SRSF2

Antigène: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Autre désignation: SC35 (SRSF2 Produits)

Synonymes: anticorps PR264, anticorps SC-35, anticorps SC35, anticorps SFRS2, anticorps SFRS2A, anticorps SRp30b, anticorps D11Wsu175e, anticorps MRF-1, anticorps Pr264, anticorps Sfrs10, anticorps Sfrs2, anticorps pr264, anticorps sc-35, anticorps sc35, anticorps sfrs2, anticorps sfrs2a, anticorps srp30b, anticorps serine and arginine rich splicing factor 2, anticorps serine/arginine-rich splicing factor 2, anticorps serine/arginine-rich splicing factor 2 L homeolog, anticorps SRSF2, anticorps Srsf2, anticorps srsf2.L

Sujet: SC35 belongs to a highly conserved SR splicing factor family. Members of this family have characteristic RNA-binding domains and serine/arginine-rich (SR) motifs. SR proteins are expressed in the nucleus and are essential pre-messenger RNA splicing factors. Nuclear pre-messenger RNA takes place in spliceosomes, multicomponent complexes consisting of five small nuclear ribonucleoprotein particles (U1, U2, U4, U5, U6) and other non-snRNP factors such as SC35. SC35 is necessary for an early step in spliceosome assembly and can also influence the selection of alternative splice sites. SC35 is identified as a 35 kDa protein in SDS/PAGE. Clone alphaSC35 has been shown to recognize SC35 in human, and rat cells. However, it should recognize SC35 from any species, as SC35 is conserved from Drosophila to man. Partially purified spliceosomes were used as immunogen.