CD247 anticorps (AA 153-164)

N° du produit ABIN967517

L’anticorps Souris Monoclonal anti-CD247 a été validé pour WB et IP. Il convient pour détecter CD247 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour CD247 anticorps (AA 153-164) (ABIN967517)

Antigène: CD247 (CD247 Molecule (CD247))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD247 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 1D4

Détail du produit anti-CD247 anticorps

Épitope: AA 153-164

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human CD3zeta synthetic peptide aa. 153-164

Isotype: IgG1

Information d'application

Indications d'application: Applications include immunoprecipitation and western blot analysis (1-2 µg/ml). Jurkat T cells (ATCC TIB 152) are suggested as a positive control.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD247 (ABIN967517)

Hall, Sancho, Terhorst: "Reconstitution of T cell receptor zeta-mediated calcium mobilization in nonlymphoid cells." dans: Science (New York, N.Y.), Vol. 261, Issue 5123, pp. 915-8, (1993) (PubMed).

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, (1993) (PubMed).

Sancho, Franco, Chatila, Hall, Terhorst: "The T cell receptor associated CD3-epsilon protein is phosphorylated upon T cell activation in the two tyrosine residues of a conserved signal transduction motif." dans: European journal of immunology, Vol. 23, Issue 7, pp. 1636-42, (1993) (PubMed).

Détails sur CD247

Antigène: CD247 (CD247 Molecule (CD247))

Autre désignation: CD247

Sujet: The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains, gamma (gamma), delta (delta), epsilon (epsilon), zeta (zeta), and p21. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer), and 21 kD respectively. 1D4 recognizes human but not mouse CD3zeta. It is most efficient at recognizing the phosphorylated form of CD3zeta (21 kD) but will also bind the 16 kD unphosphorylated monomer as well as higher molecular weight dimers. A synthetic peptide corresponding to amino acids 153 to 164 of human CD3zeta was used as immunogen.
Synonyms: CD3zeta chain

Poids moléculaire: 21 kDa

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway