PIK3R1 anticorps

N° du produit ABIN967523

L’anticorps Souris Monoclonal anti-PIK3R1 a été validé pour WB, ELISA et IP. Il convient pour détecter PIK3R1 dans des échantillons de Humain, Boeuf (Vache) et Singe. Il y a 2+ publications disponibles.

Aperçu rapide pour PIK3R1 anticorps (ABIN967523)

Antigène: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivité: Humain, Boeuf (Vache), Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PIK3R1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: U15

Détail du produit anti-PIK3R1 anticorps

Marque: BD Pharmingen™

Réactivité croisée: Humain, Singe

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant cow p85alpha

Isotype: IgG1

Information d'application

Indications d'application: Clone U15 was originally characterized by ELISA, immunoprecipitation, and western blot analysis. Applications include immunoprecipitation (1-2 µg/1x10^6 cells) and western blot analysis (1-2 µg/ml). In immunoprecipitation, U15 can bring down an active kinase. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-PIK3R1 (ABIN967523)

Shoelson, Sivaraja, Williams, Hu, Schlessinger, Weiss: "Specific phosphopeptide binding regulates a conformational change in the PI 3-kinase SH2 domain associated with enzyme activation." dans: The EMBO journal, Vol. 12, Issue 2, pp. 795-802, (1993) (PubMed).

Whitman, Downes, Keeler, Keller, Cantley: "Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate." dans: Nature, Vol. 332, Issue 6165, pp. 644-6, (1988) (PubMed).

Détails sur PIK3R1

Antigène: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Autre désignation: pi 3-Kinase p85 alpha

Sujet: Phosphatidylinositol 3-kinase (PI3-kinase) is a universal enzyme associated with receptor signaling pathways. It generates second messenger phospholipids by phosphorylating the D-3 position of inositol phospholipids including phosphatidylinositol (PI), PI-4-phosphate, and PI-4, 5-biphosphate. The enzyme exists as a heterodimer composed of regulatory 85 kDa (p85) and catalytic 110 kDa (p110) subunits. The p85 subunit contains two SH2 (src-homology 2) domains and an SH3 domain. The catalytic activity of the p110 subunit is stimulated when the p85 regulatory subunit binds, through its SH2 domains, to activated receptor and non-receptor tyrosine kinases. Two p85 isoforms have been described, p85a and p85ß. Both isoforms bind to activated receptors and each may be responsible for mediating a subset of PI3-kinase interactions. Clone U15 recognizes the p85 regulatory subunit of PI3 kinase (p85alpha). It reacts with human, monkey, and cow PI3 kinase. It does not cross-react with mouse or rat PI3 kinase. Recombinant cow p85alpha was used as immunogen. The epitope has been mapped to the inter-SH2 spacer region of p85alpha. The antibody will block lipids binding to this region.

Poids moléculaire: 85 kDa

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, L'effet Warburg