BAX anticorps (N-Term)

N° du produit ABIN967537

Détail du produit anti-BAX anticorps

Antigène: BAX (BCL2-Associated X Protein (BAX))

Épitope: N-Term

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BAX est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: N-terminal peptide sequence

Clone: 6A7

Isotype: IgG1 kappa

Information d'application

Indications d'application: Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation. Daudi B lymphoma cells (ATCC CCL-213) and rat thymocytes are suggested as positive controls for western blot analysis. Due to low level of Bax expression in Hela cells, we do not recommend Hela as a positive control.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-BAX (ABIN967537)

Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, (1997) (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." dans: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, (1997) (PubMed).

Krajewski, Krajewska, Shabaik, Miyashita, Wang, Reed: "Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2." dans: The American journal of pathology, Vol. 145, Issue 6, pp. 1323-36, (1995) (PubMed).

Reed: "Bcl-2 and the regulation of programmed cell death." dans: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, (1994) (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." dans: Nature, Vol. 369, Issue 6478, pp. 321-3, (1994) (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." dans: Cell, Vol. 74, Issue 4, pp. 609-19, (1993) (PubMed).

Détails sur BAX

Antigène: BAX (BCL2-Associated X Protein (BAX))

Autre désignation: Bax (BAX Produits)

Synonymes: anticorps BAX-ALPHA, anticorps bax-A, anticorps xBax, anticorps xlbax, anticorps BAX, anticorps bax, anticorps fj16e01, anticorps wu:fc50b10, anticorps wu:fj16e01, anticorps BCL2L4, anticorps zgc:112983, anticorps BCL2 associated X, apoptosis regulator, anticorps BCL2-associated X protein L homeolog, anticorps BCL2-associated X protein, anticorps bcl2-associated X protein, a, anticorps bcl2-associated X protein, b, anticorps BAX, anticorps bax.L, anticorps bax, anticorps baxa, anticorps Bax, anticorps baxb

Sujet: The Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax may form homodimers or heterodimers with either Bcl-2 and Bcl-XL (long). Bax homodimers is thought to promote cell death, whereas Bax heterodimerization with either Bcl-2 and Bcl-XL appears to block cell death. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. Bax expression has been identified in a variety of tissues, including lung, stomach, kidney, thymus, lymph nodes, bone marrow, spleen, heart, exocrine pancreas, and brain. Liver, kidney, and the exocrine pancreas have been found to contain little or no Bcl-2. The Bax gene potentially encodes three different proteins Bax-alpha (21 kDa), Bax-beta (24 kDa) and Bax-gamma (5 kDa). Bax-alpha is the most common transcript. The 6A7 antibody recognizes human, mouse and rat Bax. The 6A7 antibody reacts with an epitope between amino acids 12-24 of Bax, and studies suggest that this epitope may be in the vicinity of the dimerization domain of Bax. It has been proposed that the 6A7 antibody may directly compete with either Bcl-2 or Bcl-XL for binding to Bax. In western blots, the 6A7 antibody typically detects Bax as a single band of 21-22 kDa, thought to be Bax-alpha.

Poids moléculaire: 21-22 kDa

Pathways: Signalisation p53, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response