SREBF1 anticorps (AA 301-407)

N° du produit ABIN967563

Détail du produit anti-SREBF1 anticorps

Antigène: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Épitope: AA 301-407

Reactivité: Humain, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SREBF1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Réactivité croisée: Hamster

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human SREBP-1 aa. 301-407

Clone: IgG-2A4

Isotype: IgG1 kappa

Information d'application

Indications d'application: The IgG-2A4 antibody may be used for western blot analysis (0.5 to 1 µg/ml) and immunoprecipitation (2 µg/ml). U-937 human histiocytic lymphoma cells (ATCC CRL-1593) or HeLa human cervical carcinoma cells (ATCC CCL-2) are recommended as positive controls for these applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-SREBF1 (ABIN967563)

Sakai, Nohturfft, Cheng, Ho, Brown, Goldstein: "Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein." dans: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 20213-21, (1997) (PubMed).

Wang, Zelenski, Yang, Sakai, Brown, Goldstein: "Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis." dans: The EMBO journal, Vol. 15, Issue 5, pp. 1012-20, (1996) (PubMed).

Wang, Pai, Wiedenfeld, Medina, Slaughter, Goldstein, Brown: "Purification of an interleukin-1 beta converting enzyme-related cysteine protease that cleaves sterol regulatory element-binding proteins between the leucine zipper and transmembrane domains." dans: The Journal of biological chemistry, Vol. 270, Issue 30, pp. 18044-50, (1995) (PubMed).

Sato, Yang, Wang, Evans, Ho, Goldstein, Brown: "Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory element-binding protein-1 (SREBP-1)." dans: The Journal of biological chemistry, Vol. 269, Issue 25, pp. 17267-73, (1994) (PubMed).

Détails sur SREBF1

Antigène: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Autre désignation: SREBP-1 (SREBF1 Produits)

Synonymes: anticorps SREBP-1c, anticorps SREBP1, anticorps bHLHd1, anticorps ADD-1, anticorps ADD1, anticorps D630008H06, anticorps SREBP-1, anticorps SREBP-1a, anticorps SREBP1c, anticorps Srebp1, anticorps SCREBP1, anticorps SREBP-1C, anticorps SREBF1, anticorps srebp1, anticorps sterol regulatory element binding transcription factor 1, anticorps sterol regulatory element binding transcription factor 1 L homeolog, anticorps sterol regulatory element binding protein-1, anticorps Sterol regulatory element Binding Protein, anticorps SREBF1, anticorps Srebf1, anticorps srebf1.L, anticorps SREBP-1, anticorps srebf1, anticorps sbp-1

Sujet: SREBP-1 and -2 (sterol-regulatory element binding proteins-1 and -2) are transcription factors which participate in the control of cholesterol homeostasis. SREBPs proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, an ~480-500 amino acid, N-terminal cleavage fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis. Proteases which cleave SREBPs have been identified and include SCA (SREBP-cleavage activity), as well as a key regulator of apoptotic pathways, caspase-3. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes. However, sterol-regulated vs. apoptosis-associated cleavage of SREBP proteins appears to be independantly regulated. On SDS-PAGE, sterol-regulated cleavage fragments of SREBP proteins migrate more slowly (i.e., higher molecular weight) than do staurosporin-induced fragments. In addition, staurosporin-induced SREBP cleavage products may appear as a doublet, with the upper band representing a phosphorylated form of SREBP. On SDS-PAGE, full length, precursor forms of SREBP-1 and -2 migrate at ~125 kDa, while proteolytic cleavage fragments may be observed as a cluster of bands between 60 - 70 kDa. The IgG-2A4 antibody recognizes human and hamster SREBP-1. The antibody recognizes the N-terminal (basic helix-loop-helix) domain of human SREBP-1. A fusion protein containing N-terminal amino acids 301-407 (the bHLH/leucine zipper domain), was used as immunogen. The antibody recognizes both the 125 kDa precursor and 60-70 kDa mature, cleaved forms of SREBP-1.

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha