PDPK1 anticorps (pSer241)

N° du produit ABIN967612

Cet anticorps Souris Monoclonal détecte spécifiquement PDPK1 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 2+ publications.

Aperçu rapide pour PDPK1 anticorps (pSer241) (ABIN967612)

Antigène: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDPK1 est non-conjugé

Application: Western Blotting (WB)

Clone: J66-653-44-22

Détail du produit anti-PDPK1 anticorps

Épitope: pSer241

Réactivité croisée (Details): Predicted: Mouse, Rat

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human PDPK1 Peptide

Isotype: IgG1 kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-PDPK1 (ABIN967612)

Komander, Kular, Deak, Alessi, van Aalten: "Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding." dans: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 18797-802, (2005) (PubMed).

Wick, Ramos, Chen, Quon, Dong, Liu: "Mouse 3-phosphoinositide-dependent protein kinase-1 undergoes dimerization and trans-phosphorylation in the activation loop." dans: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 42913-9, (2003) (PubMed).

Détails sur PDPK1

Antigène: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Autre désignation: PDPK1

Sujet: The serine/threonine kinase 3-Phosphoinositide-Dependent Protein Kinase-1 (PDPK1, also known as PDK1) contributes to the activation of many important kinases in the insulin and IGF-1 signaling pathways. It acts downstream of phosphatidylinositol 3-kinase (PI3-kinase) to phosphorylate residues in the activation loops of many cellular kinases, including protein kinase B (PKB/Akt), PKC isoforms, p70 S6 kinase, and PDPK1 itself. The autophosphorylation of PDPK1 at serine 241 (S241) has recently been suggested to play a role in the regulation of PDPK1. It has been proposed that PDPK1 activity plays a key role in the regulation of various cellular events such as cell proliferation, differentiation, and apoptosis.
The J666-653.44.22 monoclonal antibody recognizes the phosphorylated S241 in the activation loop of human PDPK1. The orthologous phosphorylation site in mouse and rat PDPK1 is S244.
Synonyms: PDK1 (pS241), PKB Kinase (pS241)

Poids moléculaire: 63 kDa

Pathways: Signalisation PI3K-Akt, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals