Paxillin anticorps (AA 1-557)

N° du produit ABIN967711

Cet anticorps Souris Monoclonal détecte spécifiquement Paxillin dans WB, IP, IF et IHC (f). Il présente une réactivité envers Humain, Souris, Rat, Poulet et Chien et a été mentionné dans 4+ publications.

Aperçu rapide pour Paxillin anticorps (AA 1-557) (ABIN967711)

Antigène: Paxillin (PXN)

Reactivité: Humain, Souris, Rat, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Paxillin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 349-Paxillin

Détail du produit anti-Paxillin anticorps

Épitope: AA 1-557

Réactivité croisée: Humain, Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Chicken Paxillin aa. 1-557

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Paxillin (ABIN967711)

Even-Ram, Maoz, Pokroy, Reich, Katz, Gutwein, Altevogt, Bar-Shavit: "Tumor cell invasion is promoted by activation of protease activated receptor-1 in cooperation with the alpha vbeta 5 integrin." dans: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 10952-62, (2001) (PubMed).

Nikolopoulos, Turner: "Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion." dans: The Journal of cell biology, Vol. 151, Issue 7, pp. 1435-48, (2001) (PubMed).

van de Water, Houtepen, Huigsloot, Tijdens: "Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury." dans: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36183-93, (2001) (PubMed).

Wine, Chapin: "Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat." dans: Journal of andrology, Vol. 20, Issue 2, pp. 198-213, (1999) (PubMed).

Détails sur Paxillin

Antigène: Paxillin (PXN)

Autre désignation: Paxillin

Sujet: A number of cytoskeletal proteins are tyrosine phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts. One of these is the 68 kDa paxillin protein. Paxillin is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers. It is also present in the focal adhesions of Madin-Darby canine kidney epithelial cells, but is absent from the cell adherens junctions of these cells. Paxillin purified from chicken gizzard migrates as a diffuse band on SDS-PAGE with molecular weight of 65-70 kDa. It binds to the rod domain of vinculin, another focal adhesion protein. It is thought that phosphorylation of paxillin may have a role in that disassembly of focal adhesions and stress fibers during transformation. This antibody is routinely tested by Western blot analysis.

Poids moléculaire: 68 kDa

Pathways: Signalisation MAPK, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling