ARAF anticorps (AA 183-199)

N° du produit ABIN967727

L’anticorps Souris Monoclonal anti-ARAF a été validé pour WB, IHC, IF et IP. Il convient pour détecter ARAF dans des échantillons de Humain, Souris, Rat, Chien et Poulet. Il y a 4+ publications disponibles.

Aperçu rapide pour ARAF anticorps (AA 183-199) (ABIN967727)

Antigène: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ARAF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 1-A

Détail du produit anti-ARAF anticorps

Épitope: AA 183-199

Réactivité croisée: Humain, Poulet, Chien, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse A-Raf aa. 183-199

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ARAF (ABIN967727)

Gomez, Pritchard, Herbert: "cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells." dans: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48146-51, (2002) (PubMed).

Storm, Cleveland, Rapp: "Expression of raf family proto-oncogenes in normal mouse tissues." dans: Oncogene, Vol. 5, Issue 3, pp. 345-51, (1990) (PubMed).

Rapp, Heidecker, Huleihel, Cleveland, Choi, Pawson, Ihle, Anderson: "raf family serine/threonine protein kinases in mitogen signal transduction." dans: Cold Spring Harbor symposia on quantitative biology, Vol. 53 Pt 1, pp. 173-84, (1989) (PubMed).

Huleihel, Goldsborough, Cleveland, Gunnell, Bonner, Rapp: "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene." dans: Molecular and cellular biology, Vol. 6, Issue 7, pp. 2655-62, (1987) (PubMed).

Détails sur ARAF

Antigène: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Autre désignation: A-Raf

Sujet: A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 68 kDa

Pathways: Signalisation MAPK, Signalisation RTK, Hepatitis C