PKC epsilon anticorps (AA 1-175)

N° du produit ABIN967734

Détail du produit anti-PKC epsilon anticorps

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Épitope: AA 1-175

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC epsilon est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Rat (Rattus), Chien, Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human PKCepsilon aa. 1-175

Clone: 21-PKCepsilon

Isotype: IgG2a

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-PKC epsilon (ABIN967734)

Baines, Song, Zheng, Wang, Zhang, Wang, Guo, Bolli, Cardwell, Ping: "Protein kinase Cepsilon interacts with and inhibits the permeability transition pore in cardiac mitochondria." dans: Circulation research, Vol. 92, Issue 8, pp. 873-80, (2003) (PubMed).

Balafanova, Bolli, Zhang, Zheng, Pass, Bhatnagar, Tang, Wang, Cardwell, Ping: "Nitric oxide (NO) induces nitration of protein kinase Cepsilon (PKCepsilon ), facilitating PKCepsilon translocation via enhanced PKCepsilon -RACK2 interactions: a novel mechanism of no-triggered activation of PKCepsilon." dans: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15021-7, (2002) (PubMed).

Ping, Song, Zhang, Guo, Cao, Li, Wu, Vondriska, Pass, Tang, Pierce, Bolli: "Formation of protein kinase C(epsilon)-Lck signaling modules confers cardioprotection." dans: The Journal of clinical investigation, Vol. 109, Issue 4, pp. 499-507, (2002) (PubMed).

Zhu, Fang, Narla, Uckun: "A requirement for protein kinase C inhibition for calcium-triggered apoptosis in acute lymphoblastic leukemia cells." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 5, Issue 2, pp. 355-60, (1999) (PubMed).

Mischak, Kolch, Goodnight, Davidson, Rapp, Rose-John, Mushinski: "Expression of protein kinase C genes in hemopoietic cells is cell-type- and B cell-differentiation stage specific." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 11, pp. 3981-7, (1991) (PubMed).

Ohno, Akita, Hata, Osada, Kubo, Konno, Akimoto, Mizuno, Saido, Kuroki: "Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC." dans: Advances in enzyme regulation, Vol. 31, pp. 287-303, (1991) (PubMed).

Détails sur PKC epsilon

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Autre désignation: PKC epsilon (PRKCE Produits)

Sujet: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2], phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta,eta, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta , iota , and lambda isoforms) are Ca[2+] independent and lack the C2 domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Expression of the 90 kDa PKCalpha is induced by interferon-alpha. Generally, PKCalpha is expressed at very low levels in normal murine tissues, except brain. Overexpression of PKCalpha leads to increased growth rates and higher cell densities in monolayer cultures. A high level of expression is seen in several hematopoietic cell lines and tumors. This suggests a possible role for PKCalpha in tumorigenesis.

Poids moléculaire: 90 kDa

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling