SLC12A9 anticorps (AA 1-150)

N° du produit ABIN967844

Détail du produit anti-SLC12A9 anticorps

Antigène: SLC12A9 (Solute Carrier Family 12 (Potassium/chloride Transporters), Member 9 (SLC12A9))

Épitope: AA 1-150

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SLC12A9 est non-conjugé

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Cip1 aa. 1-150

Clone: 70-Cip1-WAF1

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968553, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SLC12A9 (ABIN967844)

Carrano, Pagano: "Role of the F-box protein Skp2 in adhesion-dependent cell cycle progression." dans: The Journal of cell biology, Vol. 153, Issue 7, pp. 1381-90, (2001) (PubMed).

Fima, Shtutman, Libros, Missel, Shahaf, Kahana, Livneh: "PKCeta enhances cell cycle progression, the expression of G1 cyclins and p21 in MCF-7 cells." dans: Oncogene, Vol. 20, Issue 46, pp. 6794-804, (2001) (PubMed).

Yan, Deng, Friedman: "Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III." dans: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1555-63, (2001) (PubMed).

Cheng, Lloyd, Weaver, Pisansky, Cheville, Ramnani, Leibovich, Blute, Zincke, Bostwick: "The cell cycle inhibitors p21WAF1 and p27KIP1 are associated with survival in patients treated by salvage prostatectomy after radiation therapy." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 6, Issue 5, pp. 1896-9, (2000) (PubMed).

Dulić, Stein, Far, Reed: "Nuclear accumulation of p21Cip1 at the onset of mitosis: a role at the G2/M-phase transition." dans: Molecular and cellular biology, Vol. 18, Issue 1, pp. 546-57, (1998) (PubMed).

Détails sur SLC12A9

Antigène: SLC12A9 (Solute Carrier Family 12 (Potassium/chloride Transporters), Member 9 (SLC12A9))

Autre désignation: Cip1 (SLC12A9 Produits)

Synonymes: anticorps CIP1, anticorps Ccc6, anticorps Cip1, anticorps CCC6, anticorps WO3.3, anticorps hCCC6, anticorps cip1, anticorps solute carrier family 12 (potassium/chloride transporters), member 9, anticorps solute carrier family 12, member 9, anticorps solute carrier family 12 member 9, anticorps solute carrier family 12, member 9 L homeolog, anticorps Slc12a9, anticorps SLC12A9, anticorps slc12a9.L

Sujet: Cip1 (p21) was identified as an inhibitor of cdk activity in a quaternary complex that also included Cyclin D, Cdk4, and PCNA. It has subsequently been shown that Cip1 can directly bind to and inhibit each member of the cdk family, though the affinities vary for each enzyme. Several studies indicate that Cip1 expression is regulated by the p53 tumor suppressor protein. For example, following DNA damage, Cip1 becomes transcriptionally induced in a p53-dependent manner. Thus, Cip1 protein may have a prominent role in mediating cell cycle arrest. Cip1 is also a component of active cyclin/cdk kinases. It has been suggested that Cip1-containing enzymes may transition between active and inactive states through changes in Cip1 stoichiometry. Active complexes appear to contain a single Cip1 molecule, while the inactive complexes have multiple Cip1 subunits. When multiple subunits are complexed with a cdk, cyclin, and PCNA, these Cip1 molecules can block the access of cdk-activating kinase (CAK) to cdk, thus preventing its phosphorylation and activation. However, inhibition of cdk activity by Cip1 does not appear to be dependent upon this mechanism. Other studies on DNA replication indicate that Cip1 can inhibit this process in vitro by directly binding to PCNA, a DNA polymerase-delta processivity factor.
Synonyms: WAF1

Poids moléculaire: 21 kDa