MEK2 anticorps (AA 1-110)

N° du produit ABIN967846

Cet anticorps Souris Monoclonal détecte spécifiquement MEK2 dans WB, IP, IF et IHC (f). Il présente une réactivité envers Humain, Souris, Rat, Chien, Poulet et Grenouille et a été mentionné dans 5+ publications.

Aperçu rapide pour MEK2 anticorps (AA 1-110) (ABIN967846)

Antigène: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reactivité: Humain, Souris, Rat, Chien, Poulet, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MEK2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 96-MEK2

Détail du produit anti-MEK2 anticorps

Épitope: AA 1-110

Réactivité croisée: Souris, Poulet, Chien, Grenouille, Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat MEK2 aa. 1-110

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-MEK2 (ABIN967846)

Tworkowski, Salghetti, Tansey: "Stable and unstable pools of Myc protein exist in human cells." dans: Oncogene, Vol. 21, Issue 55, pp. 8515-20, (2002) (PubMed).

Downey, Butler, Tapper, Fialkow, Saltiel, Rubin, Grinstein: "Importance of MEK in neutrophil microbicidal responsiveness." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 434-43, (1998) (PubMed).

Wu, Harrison, Dent, Lynch, Weber, Sturgill: "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." dans: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4539-48, (1993) (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." dans: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, (1992) (PubMed).

Hattori, Fukuda, Yamashita, Nakamura, Gotoh, Nishida: "Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system." dans: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20346-51, (1992) (PubMed).

Détails sur MEK2

Antigène: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Autre désignation: MEK2

Sujet: MEK2 (MAP Kinase Kinase 2 or ERK Kinase), a 46 kDa protein kinase, phosphorylates MAP kinases (ERKs) at tyrosine and threonine residues. This phosphorylation results in activation of the MAP kinases. MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase Kinase 2, ERK Kinase

Poids moléculaire: 46 kDa

Pathways: Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling