EIF2AK2 anticorps (AA 117-250)

N° du produit ABIN968165

Détail du produit anti-EIF2AK2 anticorps

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Épitope: AA 117-250

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EIF2AK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human p68 Kinase aa. 117-250

Clone: 13-PKR

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-EIF2AK2 (ABIN968165)

Feng, Chong, Kumar, Williams: "Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5447-51, (1992) (PubMed).

Lee, Tomita, Hovanessian, Katze: "Characterization and regulation of the 58,000-dalton cellular inhibitor of the interferon-induced, dsRNA-activated protein kinase." dans: The Journal of biological chemistry, Vol. 267, Issue 20, pp. 14238-43, (1992) (PubMed).

Barber, Tomita, Hovanessian, Meurs, Katze: "Functional expression and characterization of the interferon-induced double-stranded RNA activated P68 protein kinase from Escherichia coli." dans: Biochemistry, Vol. 30, Issue 42, pp. 10356-61, (1991) (PubMed).

Meurs, Chong, Galabru, Thomas, Kerr, Williams, Hovanessian: "Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon." dans: Cell, Vol. 62, Issue 2, pp. 379-90, (1990) (PubMed).

Détails sur EIF2AK2

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Autre désignation: PKR (EIF2AK2 Produits)

Synonymes: anticorps PKR, anticorps pkr, anticorps EIF2AK2, anticorps DKFZp469K1934, anticorps EIF2AK1, anticorps PRKR, anticorps 2310047A08Rik, anticorps 4732414G15Rik, anticorps AI467567, anticorps AI747578, anticorps Pkr, anticorps Prkr, anticorps Tik, anticorps eukaryotic translation initiation factor 2 alpha kinase 2, anticorps eukaryotic translation initiation factor 2-alpha kinase 2, anticorps EIF2AK2, anticorps eif2ak2, anticorps Eif2ak2

Sujet: Double stranded RNA (dsRNA) generated by most viruses during the infectious cycle is a potent stimulator of interferons. Interferons bind to cell surface receptors and stimulate the synthesis of several proteins that interfere with viral replication. One protein, 2'5'-oligo-adenylate synthetase, indirectly activates an endoribonuclease that degrades viral RNA. Another protein, p68 serine/threonine protein kinase (also known as PKR and TIK), is induced following interferon stimulation and activated by autophosphorylation in the presence of dsRNA. Upon activation, p68 phosphorylates the alpha subunit of the eukaryotic initiation factor 2 (eIF-2) resulting in inhibition of protein synthesis and, in turn, inhibition of viral replication. Evidence also suggests that p68 protein kinase inhibits proliferation and potentiates tumor suppressor function. In addition, p68 has been shown to phosphorylate Ikappa-B, thus activating NF-kappaB which induces interferon-beta gene transcription. This antibody is routinely tested by western blot analysis.
Synonyms: Protein Kinase R, p68 Kinase, TIK

Poids moléculaire: 68 kDa

Pathways: Réparation de l'ADN, ER-Nucleus Signaling, Hepatitis C