BTK anticorps (N-Term)

N° du produit ABIN968374

Cet anticorps Souris Monoclonal détecte spécifiquement BTK dans WB et IF. Il présente une réactivité envers Humain et a été mentionné dans 5+ publications.

Aperçu rapide pour BTK anticorps (N-Term) (ABIN968374)

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BTK est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 53-BTK

Détail du produit anti-BTK anticorps

Épitope: AA 2-172, N-Term

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human N-Terminal Btk aa. 2-172 Recombinant Protein

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968584, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-BTK (ABIN968374)

Shahan, Sorenson, Simpson, Kefalides, Lewis: "Tyrosine kinase activation in response to fungal spores is primarily dependent on endogenous reactive oxygen production in macrophages." dans: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10175-81, (2000) (PubMed).

Yang, Malek, Desiderio: "An SH3-binding site conserved in Bruton's tyrosine kinase and related tyrosine kinases mediates specific protein interactions in vitro and in vivo." dans: The Journal of biological chemistry, Vol. 270, Issue 35, pp. 20832-40, (1995) (PubMed).

Sideras, Müller, Shiels, Jin, Khan, Nilsson, Parkinson, Thomas, Brandén, Larsson: "Genomic organization of mouse and human Bruton's agammaglobulinemia tyrosine kinase (Btk) loci." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 12, pp. 5607-17, (1995) (PubMed).

Aoki, Isselbacher, Pillai: "Bruton tyrosine kinase is tyrosine phosphorylated and activated in pre-B lymphocytes and receptor-ligated B cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10606-9, (1994) (PubMed).

Vetrie, Vorechovský, Sideras, Holland, Davies, Flinter, Hammarström, Kinnon, Levinsky, Bobrow: "The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases." dans: Nature, Vol. 361, Issue 6409, pp. 226-33, (1993) (PubMed).

Détails sur BTK

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Autre désignation: Btk

Sujet: Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. It is a member of the Tec family of kinases which includes Tec and Itk. In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain. The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551. This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The PH domain is essential for proper activation and function of Btk. A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.

Poids moléculaire: 77 kDa

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling