PSME3 anticorps (AA 45-147)

N° du produit ABIN968402

Détail du produit anti-PSME3 anticorps

Antigène: PSME3

Épitope: AA 45-147

Reactivité: Humain, Rat, Souris, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PSME3 est non-conjugé

Application: Western Blotting (WB), BioImaging (BI)

Réactivité croisée: Rat (Rattus), Chien, Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Psme3/PA28-gamma aa. 45-147

Clone: 47-Psme3

Isotype: IgG1

Information d'application

Indications d'application: Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Commentaires:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-PSME3 (ABIN968402)

Kohda, Ishibashi, Shimbara, Tanaka, Matsuda, Kasahara et al.: "Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4923-35, (1998) (PubMed).

Détails sur PSME3

Antigène: PSME3

Autre désignation: Psme3 (PSME3 Produits)

Synonymes: anticorps AA410043, anticorps AU020960, anticorps Ki, anticorps PA28gamma, anticorps REGgamma, anticorps pa28g, anticorps Ab2-371, anticorps PA28-gamma, anticorps PA28G, anticorps REG-GAMMA, anticorps psme3b, anticorps proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki), anticorps proteasome activator subunit 3, anticorps proteasome activator subunit 3 S homeolog, anticorps Psme3, anticorps PSME3, anticorps psme3.S

Sujet: The 20S proteasome is the major constituent of the proteasomal complex that mediates degradation events, including the generation of antigenic peptides that interact with MHC class I. The 20S proteasome is a cylindrical shaped complex composed of four layers of rings, each with seven subunits. The inner rings contain alpha-subunits, while the outer rings contain beta-catalytic subunits. The constituents of the PA28 activator complex are additional subunits with specialized roles in class I-mediated antigen presentation. PA28 is a ring-shaped structure with alternating alpha- and beta-subunits. This complex binds the alpha-rings of 20S and stimulates its activity. Expression of the PA28 alpha- and beta-subunits is strongly induced by IFN-gamma. The protein product of the Psme3 gene, also known as the Ki antigen, is highly homologous to the PA28 alpha- and beta-subunits and has been designated the PA28 gamma-subunit. This protein forms a homohexamer that binds the 20S proteasome and is thought to modulate proteasome activity. The PA28 alpha- and beta-subunits are located in the cytoplasm and in the nucleus, while the gamma-subunit is almost exclusively nuclear.
Synonyms: PA28-gamma

Poids moléculaire: 36 kDa

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA