RAD9A anticorps (AA 264-370)

N° du produit ABIN968473

Cet anticorps Souris Monoclonal détecte spécifiquement RAD9A dans WB et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 5+ publications.

Aperçu rapide pour RAD9A anticorps (AA 264-370) (ABIN968473)

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAD9A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 56-hRad9

Détail du produit anti-RAD9A anticorps

Épitope: AA 264-370

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human hRAD9 aa. 264-370

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAD9A (ABIN968473)

Yoshida, Komatsu, Wang, Kufe: "c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage." dans: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3292-300, (2002) (PubMed).

Komatsu, Miyashita, Hang, Hopkins, Zheng, Cuddeback, Yamada, Lieberman, Wang: "Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and promotes apoptosis." dans: Nature cell biology, Vol. 2, Issue 1, pp. 1-6, (2000) (PubMed).

Volkmer, Karnitz: "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex." dans: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 567-70, (1999) (PubMed).

St Onge, Udell, Casselman, Davey: "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1." dans: Molecular biology of the cell, Vol. 10, Issue 6, pp. 1985-95, (1999) (PubMed).

Lieberman, Hopkins, Nass, Demetrick, Davey: "A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13890-5, (1997) (PubMed).

Détails sur RAD9A

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Autre désignation: hRad9

Sujet: Cell cycle checkpoints are regulatory mechanisms that prevent cell cycle progression in the presence of DNA damage or incompletely replicated DNA. Many of the genes required for cell-cycle arrest are also involved in DNA repair, apoptosis, and induction of transcription. In yeast and humans, hRAD9 plays a role in cell cycle arrest during the G2 phase before entry into mitosis. Phosphorylated hRAD9 is found in the nucleus after DNA damage, and forms DNA damage-responsive complexes with other putative checkpoint control proteins, such as hRAD1 and hHUS1. Expression of hRAD9 in S. pombe rad9::ura4+ cells restores resistance to the DNA synthesis inhibitor hydroxyurea and gamma rays. In addition, hRAD9 binds the anti-apoptotic proteins, Bcl-2 and Bcl-xL , and antisense hRAD9 RNA suppresses DNA-damage induced cell death. Thus, hRAD9 may be an important component of protein complexes that regulate cell cycle progression, as well as apoptosis, in response to DNA damage.

Poids moléculaire: 60 kDa

Pathways: Positive Regulation of Response to DNA Damage Stimulus