APAF1 anticorps (AA 252-445)

N° du produit ABIN968496

Cet anticorps anti-APAF1 est un anticorps Souris Monoclonal détectant APAF1 dans WB et IF. Adapté pour Humain. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour APAF1 anticorps (AA 252-445) (ABIN968496)

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 24-Apaf

Détail du produit anti-APAF1 anticorps

Épitope: AA 252-445

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Apaf-1 aa. 252-445

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-APAF1 (ABIN968496)

Li, Trudel, Wogan: "Nitric oxide-induced genotoxicity, mitochondrial damage, and apoptosis in human lymphoblastoid cells expressing wild-type and mutant p53." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10364-9, (2002) (PubMed).

Cain, Brown, Langlais, Cohen: "Caspase activation involves the formation of the aposome, a large (approximately 700 kDa) caspase-activating complex." dans: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22686-92, (1999) (PubMed).

Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." dans: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, (1999) (PubMed).

Soengas, Alarcón, Yoshida, Giaccia, Hakem, Mak, Lowe: "Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition." dans: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 156-9, (1999) (PubMed).

Slee, Harte, Kluck, Wolf, Casiano, Newmeyer, Wang, Reed, Nicholson, Alnemri, Green, Martin: "Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner." dans: The Journal of cell biology, Vol. 144, Issue 2, pp. 281-92, (1999) (PubMed).

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apaf-1

Sujet: The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1 (Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700 kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300 kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than free caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis. This antibody is routinely tested by western blot analysis.
Synonyms: Apoptotic Protease Activating Factor-1

Poids moléculaire: 130 kDa

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity