CHEK2 anticorps (AA 31-234)

N° du produit ABIN968596

Cet anticorps Souris Monoclonal détecte spécifiquement CHEK2 dans WB et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour CHEK2 anticorps (AA 31-234) (ABIN968596)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 19-Chk2

Détail du produit anti-CHEK2 anticorps

Épitope: AA 31-234

Réactivité croisée: Humain, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Chk2 aa. 31-234

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CHEK2 (ABIN968596)

Tominaga, Morisaki, Kaneko, Fujimoto, Tanaka, Ohtsubo, Hirai, Okayama, Ikeda, Nakanishi: "Role of human Cds1 (Chk2) kinase in DNA damage checkpoint and its regulation by p53." dans: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31463-7, (1999) (PubMed).

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: Chk2

Sujet: The cell cycle is regulated by multiple checkpoints that determine cell fate. Such checkpoints ensure that DNA replication and chromosomal segregation are completed with high fidelity. When DNA is damaged, specific kinases and phosphatases, key components of cell cycle checkpoints, function to arrest the cell cycle and provide the necessary time for DNA repair. For example, DNA damage induces arrest of the cell cycle at the G2 checkpoint via Wee1-mediated inhibitory phosphorylation of the kinase Cdc2. Inhibition is relieved by the Cdc25C phosphatase via Cdc2 dephosphorylation. In turn, the Chk1 and Chk2 protein kinases phosphorylate and inhibit Cdc25C, thus preventing activation of the Cdc2-cyclin B complex and entry into mitosis. Chk2, the human homolog of S. cerevisiae Rad53 and S. pombe Cds1, contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA) which is found in other cell cycle kinases. Chk2 is expressed during late G1 to M phase and is found in the nucleoplasm. Thus, Chk1 and Chk2 activity may be an important checkpoint that inhibits entry into mitosis in response to DNA damage. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 60 kDa

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire