GBF1 anticorps (AA 1266-1379)

N° du produit ABIN968745

Détail du produit anti-GBF1 anticorps

Antigène: GBF1 (Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1))

Épitope: AA 1266-1379

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GBF1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human GBF1 aa. 1266-1379

Clone: 25-GBF1

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-GBF1 (ABIN968745)

Claude, Zhao, Kuziemsky, Dahan, Berger, Yan, Armold, Sullivan, Melançon: "GBF1: A novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5." dans: The Journal of cell biology, Vol. 146, Issue 1, pp. 71-84, (1999) (PubMed).

Mansour, Herbrick, Scherer, Melançon: "Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24." dans: Genomics, Vol. 54, Issue 2, pp. 323-7, (1999) (PubMed).

Détails sur GBF1

Antigène: GBF1 (Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1))

Autre désignation: GBF1 (GBF1 Produits)

Synonymes: anticorps ARF1GEF, anticorps MGC148393, anticorps DKFZp459D2411, anticorps arf1gef, anticorps 1700083E03Rik, anticorps AI035702, anticorps AW049900, anticorps mKIAA0248, anticorps RGD1307160, anticorps golgi brefeldin A resistant guanine nucleotide exchange factor 1, anticorps Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, anticorps golgi-specific brefeldin A-resistance factor 1, anticorps GBF1, anticorps LOC100185387, anticorps gbf1, anticorps Gbf1

Sujet: The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgi-specific Brefeldin A (BFA)-resistance Factor (GBF1) is a guanine nucleotide exchange factor that contains a centrally located Sec7 domain, and a proline-rich region at the C-terminus. The Sec7 domain is commonly found in a variety of secretory proteins, and is required for activation of ARFs. GBF1 mRNA is ubiquitously expressed, and GBF1 protein localizes to the Golgi, as well as to perinuclear structures that contain COPI. BFA is a fungal heterocyclic lactone that disrupts membrane recruitment of ARFs. This action blocks protein secretion, and changes the morphology of various organelles, such as the Golgi. Overexpression of GBF1 suppresses BFA-induced changes in Golgi morphology, ARF activation, and coat protein recruitment. GBF1 interacts preferentially with ARF5 in the presence of Mg2+. Thus, GBF1 is a Golgi-specific guanine nucleotide exchange factor that activates ARFs through a mechanism that interferes with BFA action.
Synonyms: Golgi-specific Brefeldin A (BFA)-resistance Factor 1

Poids moléculaire: 206 kDa