Cofilin anticorps (AA 3-98)

N° du produit ABIN968755

Cet anticorps Souris Monoclonal détecte spécifiquement Cofilin dans WB et IF. Il présente une réactivité envers Humain, Souris, Rat et Chien et a été mentionné dans 3+ publications.

Aperçu rapide pour Cofilin anticorps (AA 3-98) (ABIN968755)

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cofilin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 32-Cofilin

Détail du produit anti-Cofilin anticorps

Épitope: AA 3-98

Réactivité croisée: Humain, Chien, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Cofilin aa. 3-98

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968540, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Cofilin (ABIN968755)

Chan, Bailly, Zebda, Segall, Condeelis: "Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion." dans: The Journal of cell biology, Vol. 148, Issue 3, pp. 531-42, (2000) (PubMed).

Moriyama, Yahara: "Two activities of cofilin, severing and accelerating directional depolymerization of actin filaments, are affected differentially by mutations around the actin-binding helix." dans: The EMBO journal, Vol. 18, Issue 23, pp. 6752-61, (2000) (PubMed).

Moriyama, Matsumoto, Nishida, Sakai, Yahara: "Nucleotide sequence of mouse cofilin cDNA." dans: Nucleic acids research, Vol. 18, Issue 10, pp. 3053, (1990) (PubMed).

Détails sur Cofilin

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Autre désignation: Cofilin

Sujet: Cell motility is a basic cellular behavior involved in embryogenesis, neurite outgrowth, wound healing, inflammation, and cancer metastasis. Filamentous actin is an essential component of cell protrusions involved in cell motility. The protrusion formation is regulated by actin polymerization and depolymerization. Cofilin is a ubiquitously expressed G- and F-actin binding protein that contains a nuclear localization signal, a C-terminal hexapeptide sequence that is identical to tropomyosin, and other regions that are homologous to actin binding proteins. In vitro, cofilin has actin severing activity and induces an increased off-rate from the pointed end of actin filaments. These activities can increase the rate of actin polymerization and depolymerization, as well as increase the number of barbed ends available for polymerization. Stimulation of MTLn3 cells with EGF leads to an increase in cofilin at the leading edge of laemellipodia, which correlates with an increase in the number of barbed ends at the leading edge. Cofilin function blocking antibodies inhibit both the appearance of these barbed ends and lamellipodial protrusion. Thus, cofilin is an actin binding protein that regulates actin filament formation during cell motility.

Poids moléculaire: 19 kDa

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events