GGA3 anticorps (AA 424-542)

N° du produit ABIN968815

Détail du produit anti-GGA3 anticorps

Antigène: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Épitope: AA 424-542

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GGA3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human GGA3 aa. 424-542

Clone: 8-GGA3

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-GGA3 (ABIN968815)

Boman, Zhang, Zhu, Kahn: "A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi." dans: Molecular biology of the cell, Vol. 11, Issue 4, pp. 1241-55, (2000) (PubMed).

DellAngelica, Puertollano, Mullins, Aguilar, Vargas, Hartnell, Bonifacino: "GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex." dans: The Journal of cell biology, Vol. 149, Issue 1, pp. 81-94, (2000) (PubMed).

Hirst, Lui, Bright, Totty, Seaman, Robinson: "A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome." dans: The Journal of cell biology, Vol. 149, Issue 1, pp. 67-80, (2000) (PubMed).

Détails sur GGA3

Antigène: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Autre désignation: GGA3 (GGA3 Produits)

Synonymes: anticorps C230037M19Rik, anticorps mKIAA0154, anticorps golgi associated, gamma adaptin ear containing, ARF binding protein 3, anticorps Gga3, anticorps GGA3

Sujet: The ADP-ribosylation factors (ARFs) are a family of small GTPases in the ARF superfamily that include ARFs and ARF-like (ARLs) proteins. At least six ARFs have been identified in humans: ARF1, ARF2, ARF3, ARF4, ARF5, and ARF6. ARFs are involved in intravesicular acidification and fusion of microsomal vesicles, endosome fusion, nuclear membrane assembly, and formation of clathrin-coated vesicles. GGAs are ARF-binding proteins that act as adaptor coat proteins associated with the Golgi complex. GGA1, GGA2, and GGA3 are homologous proteins that contain N-terminal VHS domains, a GGA and TOM homology region (GAT), and a C-terminal region homologous to the ear domain of gamma-adaptins. GGAs co-localize with Golgi markers in the TGN, and GGA3 is found present in coated vesicles and buds associated with the TGN. The GAT domain of GGA3 facilitates ARF1 binding, Golgi localization, and dissociation from ARF-regulated membranes. The C-terminal region of GGAs bind to MAP1A and rabaptin-5, which are binding partners of gamma-adaptins. Overexpression of GGAs alters the distribution of markers normally found in the TGN. Thus, GGAs are ARF binding proteins that regulate vesicle dynamics in the TGN.

Poids moléculaire: 90 kDa