PKC theta anticorps (pThr538)

N° du produit ABIN968924

L’anticorps Souris Monoclonal anti-PKC theta a été validé pour WB et IHC (f). Il convient pour détecter PKC theta dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour PKC theta anticorps (pThr538) (ABIN968924)

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC theta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 19-PKC

Détail du produit anti-PKC theta anticorps

Épitope: pThr538

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human PKCtheta Peptide

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN967738

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-PKC theta (ABIN968924)

Soderling: "Protein kinases. Regulation by autoinhibitory domains." dans: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, (1990) (PubMed).

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." dans: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).

Détails sur PKC theta

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Autre désignation: PKC theta

Sujet: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. Three categories exist, conventional PKC (cPKC), novel PKC (nPKC), and atypical PKC (aPKC). These proteins are products of multiple genes and alternative splicing and have different modes of activation. For example, cPKC's members (alpha, betaI, betaII, and gamma) are calcium activated, phospholipid-dependent serine/threonine specific enzymes which can also be activated by phorbol esters. However, the novel PKC (nPKC) subfamily members (delta , epsilon, pi, and theta isoforms) and the atypical PKC (PKC) subfamily members (zeta , í , and lambda isoforms) are Ca[2+] independent. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta RNA is readily detectable in skeletal muscle, lung, and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggests that it may have a role in growth and differentiation processes of these cells.
The 9/PKC monoclonal antibody recognizes the phosphorylated threonine 538 (pT538) of human PKCtheta.

Poids moléculaire: 79 kDa

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling