Ferritin anticorps

N° du produit ABIN99122

Détail du produit anti-Ferritin anticorps

Antigène: Ferritin (FE)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ferritin est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity against Ferritin from other tissues and species may occur but have not been specifically determined.

Pureté: Anti-FERRITIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Ferritin [Human Spleen]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: ELISA Dilution: 1:5.000 - 1:20.000
Immunohistochemistry Dilution: 1:200 - 1:1.000
Western Blot Dilution: 1:5.000 - 1:2.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Références pour anticorps anti-Ferritin (ABIN99122)

Mercer, Ohashi, Boeing, Jefferies, De Tito, Flynn, Tremel, Zhang, Wirth, Frith, Snijders, Williams, Tooze: "Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy." dans: The EMBO journal, Vol. 40, Issue 14, pp. e105985, (2021) (PubMed).

Mai, Hamaï, Hienzsch, Cañeque, Müller, Wicinski, Cabaud, Leroy, David, Acevedo, Ryo, Ginestier, Birnbaum, Charafe-Jauffret, Codogno, Mehrpour, Rodriguez: "Salinomycin kills cancer stem cells by sequestering iron in lysosomes." dans: Nature chemistry, Vol. 9, Issue 10, pp. 1025-1033, (2019) (PubMed).

Goodwin, Dowdle, DeJesus, Wang, Bergman, Kobylarz, Lindeman, Xavier, McAllister, Nyfeler, Hoffman, Murphy: "Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9." dans: Cell reports, Vol. 20, Issue 10, pp. 2341-2356, (2018) (PubMed).

Kimura, Jia, Kumar, Choi, Gu, Mudd, Dupont, Jiang, Peters, Farzam, Jain, Lidke, Adams, Johansen, Deretic: "Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy." dans: The EMBO journal, Vol. 36, Issue 1, pp. 42-60, (2017) (PubMed).

Mancias, Pontano Vaites, Nissim, Biancur, Kim, Wang, Liu, Goessling, Kimmelman, Harper: "Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis." dans: eLife, Vol. 4, (2016) (PubMed).

Mancias, Wang, Gygi, Harper, Kimmelman: "Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy." dans: Nature, Vol. 509, Issue 7498, pp. 105-9, (2014) (PubMed).

Détails sur Ferritin

Antigène: Ferritin (FE)

Autre désignation: Ferritin (FE Produits)

Synonymes: anticorps 1HCH, anticorps BEST:LD36673, anticorps CG2216, anticorps Dmel\\CG2216, anticorps FER1, anticorps FER1HCH, anticorps Fer-L, anticorps Fer1, anticorps Fer1HC, anticorps FerHCH, anticorps HCH, anticorps T21, anticorps T9, anticorps fer1, anticorps fer1hch, anticorps l(3)00451, anticorps l(3)j10B4, anticorps SFerH-1, anticorps SOF-35, anticorps SOF-5L, anticorps NV13011, anticorps ACP1, anticorps F8A5.24, anticorps F8A5_24, anticorps Ferritin 1 heavy chain homologue, anticorps ferritin light chain, anticorps ferritin, anticorps chromosome 26 open reading frame, human C10orf131, anticorps hyaluronan and proteoglycan link protein 2, anticorps NAD(P)-linked oxidoreductase superfamily protein, anticorps Fer1HCH, anticorps H-1, anticorps YPDSF_1340, anticorps ftnA, anticorps Ft, anticorps C26H10orf131, anticorps HAPLN2, anticorps AT1G60730

Sujet: Synonyms: Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

ID gène: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis