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FACS Antibodies

Le tri cellulaire activé par fluorescence (FACS) est un type spécialisé de cytométrie de flux. Il permet de trier un mélange hétérogène de cellules biologiques dans deux conteneurs ou plus, une cellule à la fois, sur la base des caractéristiques spécifiques de diffusion de la lumière et de fluorescence de chaque cellule et des anticorps qui y sont fixés.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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86.471 Résultats

TLR9 anticorps (AA 868-1016)

TLR9 Reactivité: Humain IHC, ELISA, FACS, ICC Hôte: Souris Monoclonal 1B12H2 unconjugated

Immunofluorescence validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

Proximity Ligation Assay validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

Western blot analysis using TLR9 mAb against human TLR9 (AA: 868-1016) recombinant protein.

Flow cytometric analysis of A549 cells using TLR9 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of SK-OV-3 cells using TLR9 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TLR9 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TLR9 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using TLR9 mAb against HEK293 (1) and TLR9 (AA: 868-1016)-hIgGFc transfected HEK293 (2) cell lysate.

9 images

Independent Validation

2 validations

N° du produit ABIN5542492

Independently Validated

BAX anticorps (AA 84-175)

BAX Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Lapin, Poulet, Poisson zèbre (Danio rerio) WB, ELISA, FACS, IP, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

L1 rat brain, L2 mouse liver lysates probed at 1:200 in 4˚C.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:800 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, unconjugated (ABIN725390) at 1: 800 followed by incubation with conjugated secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Human HL-60 cells probed with Bax Polyclonal Antibody, Unconjugated (green) at 1:30 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –BAX Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining

L1 rat brain, L2 mouse liver lysates probed (ABIN725390) at 1:200 in 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:800 followed by conjugation to the secondary antibody and DAB staining

11 images

34 références

N° du produit ABIN725390

CD163 anticorps (AA 1001-1121)

CD163 Reactivité: Humain, Souris, Rat, Porc, Chien WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CD163 Polyclonal Antibody, Unconjugated (ABIN741570) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Chronic phase glibenclamide reduces inflammation and improves the macrophage phenotype in EAE.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-CD163/M130 Polyclonal Antibody, PE-Cy5.

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163/M130 Polyclonal Antibody, Unconjugated (ABIN741570) followed by conjugation to the secondary antibody and DAB staining

L1 rat brain, L2 mouse uterus lysates probed (ABIN741570) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

7 images

27 références

N° du produit ABIN741570

NF-kB p65 anticorps (AA 51-100)

NFkBP65 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Poulet WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (ABIN668961), Unconjugated at 1:600 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated .

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Western blot analysis of P-Akt/Akt ratio, NF-kB and P-NF-kB proteins of rats fed either placebo, (A) B.

7 images

30 références

N° du produit ABIN668961

CD8 anticorps (AA 51-150)

CD8 Reactivité: Humain, Souris, Rat FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD8 Polyclonal Antibody .

$Quantification and representative immunohistological staining of immune cells in the fracture haematoma/callus.$ IHC (p)

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C.

Image kindly submitted by Amy Beck as part of the free sample program.

Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody at 1:5000 90min in 37˚C.

H&E and IHC staining for CD8, IL-12 and IFN-γ in subcutaneous LLC1 tumors.

7 images

26 références

N° du produit ABIN671391

HSP70 anticorps

HSP70 Reactivité: Humain WB, IHC, ELISA, IF, ICC, FACS, IEM, AA, BI Hôte: Souris Monoclonal C92F3A-5 unconjugated

Western blot analyses of the Hsp72 protein expression normalized to actin contents in the diaphragm in control animals (individual rats: young n = 4 and old emphn = 2) compared with the age-matched animals exposed to CMV for 5 days with (individual rats: young n = 3 and old n = 2) and without BGP-15 (individual rats: young n = 3 and old n = 3).

Hsp70 (C92), cell lines.

Western blot analysis of heat shock protein expression in fasted mouse livers.

Neutralization effect of an anti-heat shock protein 70 monoclonal antibody on the natural killer cell phenotype.

LRET assays of the interactions of TPR mutants of HOP with HSP70 and HSP90.

PEITC is a robust inducer of the heat shock response.

PEITC is a robust inducer of the heat shock response.

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

11 images

37 références

N° du produit ABIN361708

COL3A1 anticorps

Rockland 600-401-105-0.1 COL3A1 Reactivité: Humain, Boeuf (Vache), Porc WB, IHC, ELISA, IP, FACS, DB, FM Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C Secondary antibody: 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: ABIN925618 for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa

anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.

Immunohistochemistry of Rabbit Anti-collagen type III antibody.

(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin.

Typical electropherogram of α (III) subunits, oligomers β/γ (III), and collagen type III degradation products, released from healthy and burned skin.

9 images

5 références

Independent Validation

1 validation

N° du produit ABIN5596830

Independently Validated

ATM anticorps (pSer1981)

Rockland 200-301-400s ATM Reactivité: Humain WB, IHC, ELISA, IF, IP, FACS, FM Hôte: Souris Monoclonal 10H11-E12 unconjugated

Anti-ATM Monoclonal Antibody - Western Blot Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy Anti ATM antibody showing overlay of anti-ATM pS1981 staining.

Anti-ATM Monoclonal Antibody-Immunohistochemistry.

Anti-ATMpS1981 Flow Cytometry Flow Cytometry of Mouse anti-ATMpS1981 antibody.

5 images

124 références

N° du produit ABIN6657494

Rockland Original

SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) anticorps

Rockland 200-401-a50 SARS-CoV N Reactivité: SARS Coronavirus (SARS-CoV), SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, IHC, FM Hôte: Lapin Polyclonal unconjugated

Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody.

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2.

This immunofluorescence microscopy image shows localization of SARS-CoV-2 infection in human liver ductal organoids.

Immunofluorescence of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody.

Western blot using anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46-kDa band corresponding to the protein.

Western Blot of Rabbit Anti-SARS-CoV (N) Antibody tested on Caco-2 Cell Lysates before (mock), 24, and 48 hours post infection (hpi).

Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.

Immunohistochemistry of Rabbit Anti-SARS CoV Nucleocapsid Antibody.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein.

Immunofluorescence of Rabbit Anti-SARS CoV (N) Protein Antibody.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) ProteinVariants.

14 images

97 références

N° du produit ABIN6952544

Rockland Original

RUNX3 anticorps (AA 31-130)

RUNX3 Reactivité: Humain WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Runx3 Polyclonal Antibody, Unconjugated (ABIN1714069) at 1:200 followed by conjugation to the secondary antibody and DAB staining

5 images

Independent Validation

1 validation

N° du produit ABIN1714069

Independently Validated

TTF1 anticorps (AA 201-300)

TTF1 Reactivité: Humain, Rat, Souris WB, FACS, ICC, IF (cc) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 201-300) antibody (ABIN728713)

Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Independently Validated Antibody, image provided by Science Direct, badge number 028752.

PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min.

4 images

2 références

Independent Validation

1 validation

N° du produit ABIN728713

Independently Validated

IGF1R anticorps (AA 251-350)

IGF1R Reactivité: Humain, Souris, Rat, Chien FACS Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 251-350) antibody (ABIN726575)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

4 images

2 références

Independent Validation

1 validation

N° du produit ABIN726575

Independently Validated

CDH6 anticorps (AA 281-380)

CDH6 Reactivité: Humain WB, ELISA, FACS Hôte: Lapin Polyclonal unconjugated

Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 281-380) antibody (ABIN715286)

Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 281-380) antibody (ABIN715286)

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti K Cadherin Polyclonal Antibody, Unconjugated (ABIN715286) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Images provided by the Independent Validation Program badge number 29743.

Formalin-fixed and paraffin embedded rat brain Rabbit Anti K Cadherin Polyclonal Antibody, Unconjugated (ABIN715286) at 1:200 followed by conjugation to the secondary antibody and DAB staining

5 images

Independent Validation

1 validation

N° du produit ABIN715286

Independently Validated

FZD7 anticorps (AA 501-574)

FZD7 Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 501-574) antibody (ABIN710051)

Image provided by the Independent Validation Program (badge number 29805).

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

4 images

1 reference

Independent Validation

1 validation

N° du produit ABIN710051

Independently Validated

MTOR anticorps (AA 2436-2492)

mTOR Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody (ABIN676403)

Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated and DAPI staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

9 références

Independent Validation

1 validation

N° du produit ABIN676403

Independently Validated

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