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FACS Antibodies

Le tri cellulaire activé par fluorescence (FACS) est un type spécialisé de cytométrie de flux. Il permet de trier un mélange hétérogène de cellules biologiques dans deux conteneurs ou plus, une cellule à la fois, sur la base des caractéristiques spécifiques de diffusion de la lumière et de fluorescence de chaque cellule et des anticorps qui y sont fixés.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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78.095 Résultats :

CD8 anticorps (AA 51-150)

CD8 Reactivité: Humain, Souris, Rat FACS, IHC (fro), WB, ELISA, IHC (p), IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD8 Polyclonal Antibody .

$Quantification and representative immunohistological staining of immune cells in the fracture haematoma/callus.$ IHC (p)

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C.

Image kindly submitted by Amy Beck as part of the free sample program.

Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody at 1:5000 90min in 37˚C.

H&E and IHC staining for CD8, IL-12 and IFN-γ in subcutaneous LLC1 tumors.

7 images

26 références

N° du produit ABIN671391

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CD34 anticorps (AA 201-300)

CD34 Reactivité: Humain, Souris, Rat, Lapin FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) Hôte: Lapin Polyclonal unconjugated

Expression of cell surface marker CD34 and CD133 detected by flow cytometry

Formalin-fixed and paraffin embedded rat bile duct tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN676898) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN676898) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric adenocarcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN676898) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy3 conjugated, and DAPI staining

Lane 1: human colon carcinoma Lane 2: rat kidney lysates probed with Anti CD34 Polyclonal Antibody, Unconjugated (ABIN676898) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6.

7 images

25 références

N° du produit ABIN676898

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CD163 anticorps (AA 1001-1121)

CD163 Reactivité: Humain, Souris, Rat, Porc, Chien WB, FACS, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CD163 Polyclonal Antibody, Unconjugated (ABIN741570) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Chronic phase glibenclamide reduces inflammation and improves the macrophage phenotype in EAE.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-CD163/M130 Polyclonal Antibody, PE-Cy5.

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163/M130 Polyclonal Antibody, Unconjugated (ABIN741570) followed by conjugation to the secondary antibody and DAB staining

L1 rat brain, L2 mouse uterus lysates probed (ABIN741570) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

7 images

27 références

N° du produit ABIN741570

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BAX anticorps (AA 84-175)

BAX Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Lapin, Poulet, Poisson zèbre (Danio rerio) WB, ELISA, FACS, IP, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

L1 rat brain, L2 mouse liver lysates probed at 1:200 in 4˚C.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:800 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, unconjugated (ABIN725390) at 1: 800 followed by incubation with conjugated secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Human HL-60 cells probed with Bax Polyclonal Antibody, Unconjugated (green) at 1:30 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –BAX Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining

L1 rat brain, L2 mouse liver lysates probed (ABIN725390) at 1:200 in 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:800 followed by conjugation to the secondary antibody and DAB staining

11 images

34 références

N° du produit ABIN725390

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HSP70 anticorps

HSP70 Reactivité: Humain WB, ELISA, IHC, IF, FACS, ICC, IEM, AA, BI Hôte: Souris Monoclonal C92F3A-5 unconjugated

Western blot analyses of the Hsp72 protein expression normalized to actin contents in the diaphragm in control animals (individual rats: young n = 4 and old emphn = 2) compared with the age-matched animals exposed to CMV for 5 days with (individual rats: young n = 3 and old n = 2) and without BGP-15 (individual rats: young n = 3 and old n = 3).

Hsp70 (C92), cell lines.

Western blot analysis of heat shock protein expression in fasted mouse livers.

Neutralization effect of an anti-heat shock protein 70 monoclonal antibody on the natural killer cell phenotype.

LRET assays of the interactions of TPR mutants of HOP with HSP70 and HSP90.

PEITC is a robust inducer of the heat shock response.

PEITC is a robust inducer of the heat shock response.

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708).

11 images

37 références

N° du produit ABIN361708

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ATM anticorps (pSer1981)

ATM Reactivité: Humain WB, IHC, ELISA, FACS, FM Hôte: Souris Monoclonal 10H11-E12 unconjugated

Generation of Asciz-deficient mice.

Anti-ATMpS1981 Flow Cytometry Flow Cytometry of Mouse anti-ATMpS1981 antibody.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy Anti ATM antibody showing overlay of anti-ATM pS1981 staining.

Anti-ATM Monoclonal Antibody-Immunohistochemistry.

Anti-ATM Monoclonal Antibody - Western Blot Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.

DNA damage signaling in X-DC patient cells.

Telomere-initiated senescent cells retain active DDR foci for years after senescence establishment.

Immunostaining of γH2AX, WT1 and 5mC in patients with IgA nephropathy and controls.

Immunostaining of γH2AX, WT1 and 5mC in patients with IgA nephropathy and controls.

Immunostaining of γH2AX, WT1 and 5mC in patients with IgA nephropathy and controls.

ATM is activated by spermidine in GM00637 cells.

12 images

70 références

N° du produit ABIN6656104

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MTOR anticorps (AA 2436-2492)

mTOR Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody (ABIN676403)

Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated and DAPI staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

9 références

Independent Validation

1 validation

N° du produit ABIN676403

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Cadherin anticorps (AA 841-882)

Reactivité: Humain, Souris WB, FACS, IF (cc), IF (p), IHC (p), ELISA, IHC (fro), ICC Hôte: Lapin Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

2 références

Independent Validation

1 validation

N° du produit ABIN687682

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TTF1 anticorps (AA 201-300)

TTF1 Reactivité: Humain, Rat, Souris WB, FACS, IF (cc), ICC Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 201-300) antibody (ABIN728713)

Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Independently Validated Antibody, image provided by Science Direct, badge number 028752.

PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min.

4 images

2 références

Independent Validation

1 validation

N° du produit ABIN728713

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IGF1R anticorps (AA 251-350)

IGF1R Reactivité: Humain, Souris, Rat, Chien FACS Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 251-350) antibody (ABIN726575)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

4 images

2 références

Independent Validation

1 validation

N° du produit ABIN726575

Fiche technique

Fiche de données

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