ATM anticorps (pSer1981)

N° du produit ABIN6657494

Détail du produit anti-ATM anticorps

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Épitope: AA 1974-1988, pSer1981

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Réactivité croisée: Humain, Souris, Rat (Rattus)

Réactivité croisée (Details): Cross reactivity with ATM from other mammalian sources has not been tested.

Purification: Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000, whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human and mouse ATM.

Immunogène:

Immunogen: Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Clone: 10H11-E12

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry Dilution: Not Recommended

Application Note: Protein A Purified Mab anti-ATM has been tested by ELISA and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and mouse cells. By immunofluorescence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8 (p/n 200-301-500).

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:200 - 1:2,000

IF Microscopy Dilution: 1:100 - 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Références pour anticorps anti-ATM (ABIN6657494)

Kreis, Gallrein, Rojas-Puente, Mack, Kroon, Dinkel, Willmes, Murk, Tom-Dieck, Schuman, Kirstein, Eickholt: "ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans." dans: Nature communications, Vol. 10, Issue 1, pp. 486, (2019) (PubMed).

Carvalho, Vítor, Sridhara, Martins, Raposo, Desterro, Ferreira, de Almeida: "SETD2 is required for DNA double-strand break repair and activation of the p53-mediated checkpoint." dans: eLife, Vol. 3, pp. e02482, (2015) (PubMed).

Bartkova, Horejsí, Koed, Krämer, Tort, Zieger, Guldberg, Sehested, Nesland, Lukas, Ørntoft, Lukas, Bartek: "DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis." dans: Nature, Vol. 434, Issue 7035, pp. 864-70, (2005) (PubMed).

Détails sur ATM

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Autre désignation: ATM (ATM Produits)

Synonymes: anticorps ATM, anticorps Atm, anticorps CG6535, anticorps Dmel\\CG6535, anticorps Tefu, anticorps atm, anticorps atm/tefu, anticorps dATM, anticorps tef, anticorps Xatm, anticorps at1, anticorps atdc, anticorps tel1, anticorps telo1, anticorps AT1, anticorps ATA, anticorps ATC, anticorps ATD, anticorps ATDC, anticorps ATE, anticorps TEL1, anticorps TELO1, anticorps AI256621, anticorps C030026E19Rik, anticorps telomere fusion, anticorps ATM serine/threonine kinase L homeolog, anticorps ATM serine/threonine kinase, anticorps ataxia telangiectasia mutated, anticorps ataxia telangiectasia mutated (atm), anticorps serine/threonine-protein kinase ATM, anticorps tefu, anticorps atm.L, anticorps atm, anticorps ATM, anticorps EDI_100660, anticorps CpipJ_CPIJ001772, anticorps BDBG_08252, anticorps PAAG_02532, anticorps MCYG_05088, anticorps VDBG_06833, anticorps ACLA_015700, anticorps LOC5565620, anticorps MGYG_07634, anticorps PGTG_14279, anticorps Atm

Sujet:

Synonyms: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Background: Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.

Gene Name: ATM

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus