anti-Primate TUBB anticorps pour Immunofluorescence

Recommended TUBB Antibody

Antigène
Tubulin, beta (TUBB) Anticorps
  • M40
  • OK/SW-cl.56
  • TUBB1
  • TUBB5
  • XLOT
  • m40
  • ok/sw-cl.56
  • tubb1
  • tubb5
  • TUBB2A
  • TUBB2B
  • TUBB
  • 1t
  • B1t
  • BETA 56D
  • CG9277
  • DTB2
  • Dmbeta1
  • Dmel\\CG9277
  • T
  • Tub
  • Tubulin
  • beta
  • beta-Tub
  • beta-Tub56D
  • beta-tub
  • beta-tubulin56D
  • beta1
  • beta1-Tubulin
  • beta1-tub
  • beta1Tub
  • beta1t
  • beta1tub
  • beta56D
  • betaTub
  • betaTub1
  • beta[[1]] tubulin
  • beta[[1]]-tubulin
  • betatub(56D)
  • 143391_i_at
  • 3t
  • B3t
  • BETA 60D
  • CG3401
  • D.m.BETA-60D
  • DTB3
  • Dmbeta3
  • Dmel\\CG3401
  • Tub60D
  • beta-Tub60D
  • beta-Tub6D
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3TUB
  • beta3Tub
  • beta3t
  • beta60C
  • betaTub3
  • betaTub60C
  • beta[[3]] tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • betatub60D
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • 2t
  • B2t
  • BETA 85D
  • BETA2
  • CG9359
  • D.m.BETA-85D
  • DTB4
  • Dmbeta2
  • Dmel\\CG9359
  • beta(2)Tu
  • beta(2)Tub
  • beta-Tub85D
  • beta-tub85D
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta85D
  • betaTub2
  • beta[[2]]-tubulin
  • ms(3)KK[D]
  • 4t
  • B4t
  • BETA 98B
  • CG4869
  • DTB1
  • Dmbeta4
  • Dmel\\CG4869
  • beta-Tub97EF
  • beta4
  • beta4t
  • beta97F
  • betaTub4
  • betaTub98BC
  • betaTub98C
  • Tub1
  • bmtub1
  • Tub3
  • bmtub3
  • Tub2
  • bmtub2
  • Tub4
  • bmtub4
  • LOC100101153
  • tubulin beta class I
  • putative cobalt-zinc-cadmium outer membrane resistance protein
  • Tubulin beta chain
  • tubulin beta-2A chain
  • beta-Tubulin at 56D
  • beta-Tubulin at 60D
  • beta-Tubulin at 85D
  • beta-Tubulin at 97EF
  • beta-tubulin
  • beta tubulin
  • tubulin beta class I L homeolog
  • tubulin, beta 5 class I
  • TUBB
  • czcC
  • tbb-6
  • tubb
  • LOC462399
  • betaTub56D
  • betaTub60D
  • betaTub85D
  • betaTub97EF
  • Tub1
  • Tub3
  • Tub2
  • Tub4
  • TVAG_525430
  • TVAG_062880
  • TVAG_456920
  • LOC100101153
  • Tb927.1.2330
  • Tb927.1.2350
  • Tb927.1.2370
  • Tb927.1.2390
  • tubb.L
  • Tubb
  • LOC693049
  • LOC443015
  • Tubb5
Épitope
N-Term, AA 1-100
26
18
15
15
8
7
6
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Poulet, Chinese Hamster, Boeuf (Vache), Humain, Invertébré, Souris, Porc, Primate, Rat (Rattus), Xenopus laevis, Poisson zèbre (Danio rerio)
330
226
211
34
34
27
27
25
19
18
15
15
10
9
8
7
7
6
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Hôte
Lapin
188
185
1
1
1
1
Clonalité
Polyclonal
Conjugué
Cet anticorp TUBB est non-conjugé
11
10
8
8
8
8
8
8
6
3
3
3
3
3
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
283
103
95
79
59
41
34
22
22
8
7
6
3
2
2
1
1
1
Options

Images

N° du produit ABIN269948
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
9.520397 ABIN4284184 ELISA FACS ICC IF IHC WB Mouse IgG2a N-Term BT7R 0
-5.5733232 ABIN269677 ICC IF IP ELISA WB Mouse IgG1 D66 2
-13.073323 ABIN4284200 ELISA ICC IF IHC WB Biotin Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284198 ELISA FACS ICC IF IHC WB Alexa Fluor 647 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284202 ELISA FACS ICC IF IHC WB DyLight 650 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284204 ELISA FACS ICC IF IHC WB DyLight 680 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284206 ELISA FACS ICC IF IHC WB DyLight 755 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284197 ELISA FACS ICC IF IHC WB Alexa Fluor 488 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284196 ELISA FACS ICC IF IHC WB Alexa Fluor 405 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284199 ELISA FACS ICC IF IHC WB Alexa Fluor 700 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284203 ELISA FACS ICC IF IHC FITC Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284205 ELISA FACS ICC IF IHC WB DyLight 488 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284207 ELISA FACS ICC IF IHC (p) WB PerCP Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284209 ELISA FACS ICC IF IHC WB DyLight 550 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284210 ELISA FACS ICC IF IHC WB DyLight 350 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN4284211 ELISA FACS ICC IF IHC WB DyLight 405 Mouse IgG2a N-Term BT7R 0
-13.073323 ABIN446323 ICC IF IHC IHC (p) WB Biotin Rabbit N-Term, AA 1-100 Polyclonal 0
-13.073323 ABIN4957136 ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 1-100, N-Term Polyclonal 0
-13.073323 ABIN4957138 ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 1-100, N-Term Polyclonal 0
-13.073323 ABIN4957135 ICC IF IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 1-100, N-Term Polyclonal 0

General

Antigène Tubulin, beta (TUBB) Anticorps
Épitope N-Term, AA 1-100
(26), (18), (15), (15), (8), (7), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Poulet, Chinese Hamster, Boeuf (Vache), Humain, Invertébré, Souris, Porc, Primate, Rat (Rattus), Xenopus laevis, Poisson zèbre (Danio rerio)
(330), (226), (211), (34), (34), (27), (27), (25), (19), (18), (15), (15), (10), (9), (8), (7), (7), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Hôte Lapin
(188), (185), (1), (1), (1), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp TUBB est non-conjugé
(11), (10), (8), (8), (8), (8), (8), (8), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(283), (103), (95), (79), (59), (41), (34), (22), (22), (8), (7), (6), (3), (2), (2), (1), (1), (1)
Pubmed 37 références disponible
Fournisseur

Détail du produit anti-TUBB anticorps

Détail du antigène TUBB Information d'application Stockage References for anti-TUBB antibody (ABIN269948) Images
Purification Ammonium sulfate precipitation
Immunogène A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot P07437]
Plasmids, Primers & others

Détail du antigène TUBB

Détail du produit anti-TUBB anticorps Information d'application Stockage References for anti-TUBB antibody (ABIN269948) Images Haut de la page
Antigène
Autre désignation beta Tubulin (TUBB Antibody Extrait)
Sujet Gene Symbol: TUBB
Poids moléculaire Theoretical MW: 50 kDa
ID gène 203068
UniProt P07437
Pathways Dynamique des Microtubules, M Phase

Information d'application

Détail du produit anti-TUBB anticorps Détail du antigène TUBB Stockage References for anti-TUBB antibody (ABIN269948) Images Haut de la page
Indications d'application Western Blot 1:1000, Simple Western 1:1000, Immunohistochemistry 1:1000 - 1:2000, Immunocytochemistry/Immunofluorescence 1:500-1:1000, Immunohistochemistry-Paraffin 1:1000 - 1:2000In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Protocol specific for beta Tubulin Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-beta tubulin primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Stockage

Détail du produit anti-TUBB anticorps Détail du antigène TUBB Information d'application References for anti-TUBB antibody (ABIN269948) Images Haut de la page
Format Liquid
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TUBB antibody (ABIN269948)

Détail du produit anti-TUBB anticorps Détail du antigène TUBB Information d'application Stockage Images Haut de la page
Produit citée dans:

Castle, Daude, Gilch, Westaway: "Application of high-throughput, capillary-based Western analysis to modulated cleavage of the cellular prion protein." dans: The Journal of biological chemistry, Vol. 294, Issue 8, pp. 2642-2650, 2019 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Mouse (Murine)).

Hannen, Steffani, Voellger, Carl, Wang, Bartsch, Nimsky: "Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study." dans: The Journal of steroid biochemistry and molecular biology, Vol. 187, pp. 88-96, 2019 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Rat (Rattus)).

Lee, Jun, Kim, Kim: "Astrocytic cystine/glutamate antiporter is a key regulator of erythropoietin expression in the ischemic retina." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, Issue 5, pp. 6045-6054, 2019 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Zhang, Wu, Yang, Liu, Yang, Yuan, Liu, Sun, Yang: "Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload." dans: Neuroscience, Vol. 411, pp. 86-104, 2019 Méthode utilisée: Immunofluorescence (fixed cells) (IF/ICC) (Échantillon (espèces): Rat (Rattus)).

Hannen, Selmansberger, Hauswald, Pagenstecher, Nist, Stiewe, Acker, Carl, Nimsky, Bartsch: "Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor." dans: Cancers, Vol. 11, Issue 7, 2019 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Gyöngyösi, Lukovic, Zlabinger, Spannbauer, Gugerell, Pavo, Traxler, Pils, Maurer, Jakab, Riesenhuber, Pircher, Winkler, Bergler-Klein: "Liposomal doxorubicin attenuates cardiotoxicity via induction of interferon-related DNA damage resistance." dans: Cardiovascular research, 2019 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Pig (Porcine)).

Scharfenberg, Helbig, Sammel, Benzel, Schlomann, Peters, Wichert, Bettendorff, Schmidt-Arras, Rose-John, Moali, Lichtenthaler, Pietrzik, Bartsch, Tholey, Becker-Pauly: "Degradome of soluble ADAM10 and ADAM17 metalloproteases." dans: Cellular and molecular life sciences : CMLS, 2019

Schulte, Fu, Zhao, Li, Geng, Smith, Kondo, Coffey, Johnson, Rathmell, Sharick, Skala, Smith, Berlin, Washington, Nickels, Manning: "Pharmacological blockade of ASCT2-dependent glutamine transport leads to antitumor efficacy in preclinical models." dans: Nature medicine, Vol. 24, Issue 2, pp. 194-202, 2018

Mao, Yang, Lu, Tan, Wang, Ding, Xiao, Qi, Yu: "C1QTNF3 in the murine ovary and its function in folliculogenesis." dans: Reproduction (Cambridge, England), Vol. 155, Issue 4, pp. 333-346, 2018

Yang, Chen, Lu, Tan, Chen, Li, Peng, Yuan, Cai, Yu: "Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice." dans: Reproduction (Cambridge, England), Vol. 156, Issue 2, pp. 133-144, 2018

Stuart, Fonseca, Moradi, Cunningham, Seliman, Worsfold, Dolan, Abando, Maddalena: "How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions." dans: Oxidative medicine and cellular longevity, Vol. 2018, pp. 8238459, 2018 Méthode utilisée: Western Blotting (WB)

Gelmedin, Morel, Hahnel, Cailliau, Dissous, Grevelding: "Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival." dans: PLoS pathogens, Vol. 13, Issue 1, pp. e1006147, 2017 Méthode utilisée: Immunofluorescence (fixed cells) (IF/ICC)

Ali, Lee, Chen, Chen, Lee, Shiau, Chiang, Huang: "Novel Anthra[1,2-c][1,2,5]Thiadiazole-6,11-Diones as Promising Anticancer Lead Compounds: Biological Evaluation, Characterization & Molecular Targets Determination." dans: PLoS ONE, Vol. 11, Issue 4, pp. e0154278, 2016 Méthode utilisée: Immunohistochemistry (IHC)

Snyder, Lau, Brouhard, Davis, Jiang, Sifuentes, Csankovszki: "Anchoring of Heterochromatin to the Nuclear Lamina Reinforces Dosage Compensation-Mediated Gene Repression." dans: PLoS genetics, Vol. 12, Issue 9, pp. e1006341, 2016

Lee, Huang, Chang, Huang, Hsieh, Chen, Chou, Chiang, Lai, Shiau: "Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions." dans: Scientific reports, Vol. 6, pp. 29311, 2016

Lau, Zhu, Brouhard, Davis, Csankovszki: "An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males." dans: Epigenetics & chromatin, Vol. 9, pp. 44, 2016 (Échantillon (espèces): Human).

Chen, Cheng, Tzen, Cheng, Lin: "Probing relevant molecules in modulating the neurite outgrowth of hippocampal neurons on substrates of different stiffness." dans: PLoS ONE, Vol. 8, Issue 12, pp. e83394, 2014 (Échantillon (espèces): Mouse (Murine)). Détails: Immunocytochemistry,Immunofluorescence

McKinley, Zhao, Coffey, Washington, Manning: "3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer." dans: PLoS ONE, Vol. 9, Issue 9, pp. e108193, 2014 (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Jacek, Fallon, Chandra, Crow, Wormser, Alaedini: "Increased IFNα activity and differential antibody response in patients with a history of Lyme disease and persistent cognitive deficits." dans: Journal of neuroimmunology, Vol. 255, Issue 1-2, pp. 85-91, 2013 (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

McKinley, Smith, Zhao, Fu, Saleh, Uddin, Washington, Coffey, Manning: "3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer." dans: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 54, Issue 3, pp. 424-30, 2013 (Échantillon (espèces): Human). Détails: Western Blotting

Images

Détail du produit anti-TUBB anticorps Détail du antigène TUBB Information d'application Stockage References for anti-TUBB antibody (ABIN269948) Haut de la page
Images (Fournisseur)
Immunofluorescence (IF) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [ABIN269948] - HeLa cel...
Western Blotting (WB) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Western Blot: beta Tubulin Antibody [ABIN269948] - Western blot analysis of extracts ...
Immunofluorescence (IF) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [ABIN269948] - IF analy...
Simple Western (SimWes) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Simple Western: beta Tubulin Antibody [ABIN269948] - Simple Western lane view shows a...
Western Blotting (WB) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Western Blot: beta Tubulin Antibody [ABIN269948] - Analysis in HeLa whole cell lysate...
Immunofluorescence (IF) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [ABIN269948] - Confocal...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Immunohistochemistry-Paraffin: beta Tubulin Antibody [ABIN269948] - IHC analysis of f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Immunohistochemistry-Paraffin: beta Tubulin Antibody [ABIN269948] - IHC analysis of f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Immunohistochemistry-Paraffin: beta Tubulin Antibody [ABIN269948] - IHC analysis of f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tubulin, beta (TUBB) (AA 1-100), (N-Term) antibody (ABIN269948) Immunohistochemistry-Paraffin: beta Tubulin Antibody [ABIN269948] - IHC analysis of f...