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Envoyez-nous votre proposition de validation. J'aimerais valider ce produitRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Fournisseur | Clonality | References | Details |
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1 | ABIN4952690 | ELISA FACS ICC IF IHC (p) WB | Goat | Ig Fraction | Connectez-vous pour afficher | Polyclonal | 0 |
General |
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Antigène | Sprouty Homolog 1, Antagonist of FGF Signaling (Drosophila) (SPRY1) Anticorps |
Épitope | C-Term Alternatives |
Reactivité | Humain, Souris Alternatives |
Hôte | Chèvre Alternatives |
Clonalité | |
Conjugué | Cet anticorp SPRY1 est non-conjugé Alternatives |
Application |
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
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Fournisseur | Connectez-vous pour afficher |
Détail du produit anti-SPRY1 anticorpsDétail du antigène SPRY1 Information d'application Stockage Images |
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Fonction | Sprouty |
Blocking Peptide | Blocking peptide for this product available: ABIN2816486 |
Séquence | CPSRGQGKPS |
Specificité | NP_005832.1 and NP_955359.1 represent varients of the same protein. |
Homologie | Human |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Classe de qualité | Verified |
Immunogène | Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1. |
Plasmids, Primers & others | |
Détail du antigène SPRY1Détail du produit anti-SPRY1 anticorps Information d'application Stockage Images Haut de la page |
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Antigène | |
Autre désignation | SPRY1 (SPRY1 Antibody Extrait) |
Sujet | SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling) |
ID gène | 10252 |
NCBI Accession | NP_005832, NP_955359 |
Pathways | EGFR Signaling Pathway, EGFR Downregulation |
Information d'applicationDétail du produit anti-SPRY1 anticorps Détail du antigène SPRY1 Stockage Images Haut de la page |
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Indications d'application |
DS WB Results: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162). Recommended concentration: 1-3 μg/mL. DS IHC Results: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL. Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:16000. |
Commentaires |
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. |
Restrictions | For Research Use only |
StockageDétail du produit anti-SPRY1 anticorps Détail du antigène SPRY1 Information d'application Images Haut de la page |
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Format | Liquid |
Concentration | 0.5 mg/mL |
Buffer | Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin. |
Agent conservateur | Sodium azide |
Précaution d'utilisation | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Conseil sur la manipulation | Minimize freezing and thawing. |
Stock | -20 °C |
Stockage commentaire | Aliquot and store at -20 °C. |
ImagesDétail du produit anti-SPRY1 anticorps Détail du antigène SPRY1 Information d'application Stockage Haut de la page |
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Images (Fournisseur) |