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Souris IgG1 isotype control (APC)

FACS, ELISA, FM, FLISA Monoclonal IgG1 APC
N° du produit ABIN5608794
  • Antigène Tous les produits IgG1
    IgG1
    Reactivité
    Souris
    Hôte
    • 214
    • 42
    • 27
    • 9
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Souris
    Clonalité
    • 177
    • 11
    • 6
    Monoclonal
    Conjugué
    APC
    Application
    Flow Cytometry (FACS), ELISA, Fluorescence Microscopy (FM), FLISA
    Purification
    Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG2a, IgG2b , IgG3 , IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition has been confirmed.
    Isotype
    IgG1
    Labeling Ratio
    1-2
  • Indications d'application

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG1 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

    FLISA Dilution: User Optimized

    ELISA Dilution: 1:2000-1:20,000

    IF Microscopy Dilution: User Optimized

    Commentaires

    Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 3 and μ - IgM, light chain κ and λ.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Stabilizer: None

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
    Date de péremption
    12 months
  • Antigène
    IgG1
    Abstract
    IgG1 Produits
    Synonymes
    IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control
    Classe de substances
    Antibody
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