IL-6 Kit ELISA

N° du produit ABIN1112654

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris IL-6.

Aperçu rapide pour IL-6 Kit ELISA (ABIN1112654)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Tissue Lysate

Détail du produit IL-6 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: For quantitative detection of IL-6 in mouse serum, body fluids, tissue lysates or cell culture supernates.

Analytical Method: Quantitative

Sensibilité: < 1 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-mouse IL-6 antibody 2. Lyophilized Mouse IL-6 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse IL-6 antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-6 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-6 can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysate and cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C° C.
Serum: Coagulate the serum at room temperature (about 4 hours) or coat at 4°C overnight. Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6

Sujet: Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. It acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is one of the most important mediators of fever and of the acute phase response. It is capable of crossing the blood brain barrier and initiating synthesis of PGE2 in the hypothalamus, thereby changing the body's temperature setpoint. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. IL-6 is relevant to many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, prostate cancer, and rheumatoid arthritis.

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome