FGFR1 Kit ELISA

N° du produit ABIN1569841

Détail du produit FGFR1 Kit ELISA

Antigène: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FGFR1 in human tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.057 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Autre désignation: FGFR1 (FGFR1 Produits)

Synonymes: FGFR Kit ELISA, fgfr Kit ELISA, X1FGFR Kit ELISA, XFGFR-1 Kit ELISA, XFGFRA2 Kit ELISA, bfgfr Kit ELISA, cek Kit ELISA, fgfr1 Kit ELISA, flg Kit ELISA, flt-2 Kit ELISA, flt2 Kit ELISA, kal2 Kit ELISA, ogd Kit ELISA, xfgfr1 Kit ELISA, CG7223 Kit ELISA, CT22273 Kit ELISA, CT39172 Kit ELISA, DFGF-R1 Kit ELISA, DFGF-R2 Kit ELISA, DFR-1 Kit ELISA, DFR1 Kit ELISA, DFR1/DFGF-R2 Kit ELISA, DPR3 Kit ELISA, DTRK(FR1) Kit ELISA, Dfr-1 Kit ELISA, Dfr1 Kit ELISA, DmHD-38 Kit ELISA, Dmel\\CG7223 Kit ELISA, Dtk1 Kit ELISA, EMS2 Kit ELISA, FGF-R2 Kit ELISA, FGFR2 Kit ELISA, FR1 Kit ELISA, Fr1 Kit ELISA, HD-38 Kit ELISA, HTL/FGFR1 Kit ELISA, Htl Kit ELISA, Tk1 Kit ELISA, dtk1 Kit ELISA, i100 Kit ELISA, i150 Kit ELISA, i79 Kit ELISA, j372 Kit ELISA, cd331 Kit ELISA, fgfbr Kit ELISA, fgfr-1 Kit ELISA, hbgfr Kit ELISA, n-sam Kit ELISA, ach Kit ELISA, cd333 Kit ELISA, cek2 Kit ELISA, fgfr-3 Kit ELISA, hsfgfr3ex Kit ELISA, jtk4 Kit ELISA, BFGFR Kit ELISA, CD331 Kit ELISA, CEK Kit ELISA, FGFBR Kit ELISA, FGFR-1 Kit ELISA, FLG Kit ELISA, FLT-2 Kit ELISA, FLT2 Kit ELISA, HBGFR Kit ELISA, HH2 Kit ELISA, KAL2 Kit ELISA, N-SAM Kit ELISA, OGD Kit ELISA, bFGF-R-1 Kit ELISA, AW208770 Kit ELISA, Eask Kit ELISA, Fgfr-1 Kit ELISA, Flt-2 Kit ELISA, Hspy Kit ELISA, FLG-1 Kit ELISA, FGFRI Kit ELISA, x1fgfr Kit ELISA, cb231 Kit ELISA, sb:cb231 Kit ELISA, fibroblast growth factor receptor Kit ELISA, fibroblast growth factor receptor 1 S homeolog Kit ELISA, basic fibroblast growth factor receptor 1 Kit ELISA, heartless Kit ELISA, fibroblast growth factor receptor 1 Kit ELISA, fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) L homeolog Kit ELISA, Fibroblast growth factor receptor 1 Kit ELISA, fibroblast growth factor receptor 1 L homeolog Kit ELISA, fibroblast growth factor receptor 1a Kit ELISA, FGFR Kit ELISA, fgfr Kit ELISA, fgfr1.S Kit ELISA, CpipJ_CPIJ005849 Kit ELISA, htl Kit ELISA, fgfr1 Kit ELISA, fgfr3.L Kit ELISA, FGFR1 Kit ELISA, Fgfr1 Kit ELISA, fgfr1.L Kit ELISA, fgfr1a Kit ELISA

Sujet: Alternative name: CD331, FLT2, KAL2, CEK, FLG, BFGFR, N-SAM,Basic Fibroblast Growth Factor Receptor 1, Fms-Related Tyrosine Kinase-2, Pfeiffer Syndrome

ID gène: 2260

UniProt: P11362

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins