PSMA1 Kit ELISA

N° du produit ABIN1572050

Détail du produit PSMA1 Kit ELISA

Antigène: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PSMa1 in human tissue homogenates and other biological fluids.

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.067 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Autre désignation: PSMa1 (PSMA1 Produits)

Synonymes: PSMA1 Kit ELISA, pros30 Kit ELISA, DDBDRAFT_0204226 Kit ELISA, DDBDRAFT_0214956 Kit ELISA, DDB_0204226 Kit ELISA, DDB_0214956 Kit ELISA, C2 Kit ELISA, HC2 Kit ELISA, Pros-30 Kit ELISA, alpha-type Kit ELISA, NU Kit ELISA, PROS30 Kit ELISA, CC2 Kit ELISA, zgc:92726 Kit ELISA, 143314_at Kit ELISA, Alpha-6 Kit ELISA, CG4904 Kit ELISA, Dmel\CG4904 Kit ELISA, PROS-35 Kit ELISA, PROS-Dm35 Kit ELISA, Pros-35 Kit ELISA, Pros-Dm35 Kit ELISA, Prosalpha6 Kit ELISA, alpha6 Kit ELISA, alpha_dm Kit ELISA, anon-SAGE:Wang-116 Kit ELISA, pros 35 Kit ELISA, pros35 Kit ELISA, proteasome subunit alpha 1 Kit ELISA, proteasome subunit alpha type 1 Kit ELISA, 20S proteasome subunit C2 Kit ELISA, proteasome (prosome, macropain) subunit, alpha type 1 Kit ELISA, proteasome subunit alpha 1 L homeolog Kit ELISA, Proteasome alpha6 subunit Kit ELISA, PSMA1 Kit ELISA, psma1 Kit ELISA, CNB05540 Kit ELISA, psmA1 Kit ELISA, Psma1 Kit ELISA, psma1.L Kit ELISA, Prosalpha6 Kit ELISA

Sujet: Alternative name: HC2, NU, PROS30, Proteasome(Prosome,Macropain)Subunit,Alpha Type,1, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome nu chain

ID gène: 5682

UniProt: P25786

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA