VEGFR2/CD309 Kit ELISA

N° du produit ABIN1672753

Détail du produit VEGFR2/CD309 Kit ELISA

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Épitope: AA 20-764

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human VEGFR2/KDR

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A20-E764

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <4pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A20-E764

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Tissue Specificity: Detected in cornea (at protein level). Widely expressed. .

Plaque: Pre-coated

Protocole: human VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR2 has been precoated onto 96-well plates. Standards(NSO, A20-E764) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGFR2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human VEGFR2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human VEGFR2 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 197, Standard deviation: 9.65, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 1466, Standard deviation: 51.31, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 2458, Standard deviation: 127.8, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 234, Standard deviation: 17.55, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 1630, Standard deviation: 114.1, CV(%): 7
• Sample 3: n=24, Mean(pg/ml): 2863, Standard deviation: 177.5, CV(%): 6.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour VEGFR2/CD309 Kit ELISA (ABIN1672753)

Zhang, Wang, Zhang, Yao, Zhao, Liu, Liu, Tao, Yu, Li, Song, Yao: "eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation." dans: Oncotarget, Vol. 8, Issue 12, pp. 19968-19979, (2017) (PubMed).

Bozduman, Ersoy Evans, Karahan, Hayran, Akbiyik, Lay: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." dans: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).

Wang, Liu, Wang, Wang: "Correlation of the expression of vascular endothelial growth factor and its receptors with microvessel density in ovarian cancer." dans: Oncology letters, Vol. 6, Issue 1, pp. 175-180, (2013) (PubMed).

Détail du antigène

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Autre désignation: KDR (VEGFR2 Produits)

Synonymes: CD309 Kit ELISA, FLK1 Kit ELISA, VEGFR Kit ELISA, VEGFR2 Kit ELISA, 6130401C07 Kit ELISA, Flk-1 Kit ELISA, Flk1 Kit ELISA, Krd-1 Kit ELISA, Ly73 Kit ELISA, VEGFR-2 Kit ELISA, sVEGFR-2 Kit ELISA, Vegfr-2 Kit ELISA, FLK-1 Kit ELISA, flk-1 Kit ELISA, vegfr-2 Kit ELISA, KDR Kit ELISA, FLK/KDR Kit ELISA, flk1 Kit ELISA, flk1b Kit ELISA, kdrb Kit ELISA, si:busm1-205d10.1 Kit ELISA, si:ch211-254j6.1 Kit ELISA, si:ch211-278f21.4 Kit ELISA, wu:fc31a09 Kit ELISA, fk52c05 Kit ELISA, flk Kit ELISA, kdr Kit ELISA, kdra Kit ELISA, vegfr2 Kit ELISA, vegfr4 Kit ELISA, vegr2 Kit ELISA, wu:fk52c05 Kit ELISA, kinase insert domain receptor Kit ELISA, kinase insert domain protein receptor Kit ELISA, vascular endothelial growth factor receptor kdr-like Kit ELISA, kinase insert domain receptor (a type III receptor tyrosine kinase) Kit ELISA, kinase insert domain receptor like Kit ELISA, KDR Kit ELISA, Kdr Kit ELISA, LOC100136647 Kit ELISA, kdr Kit ELISA, kdrl Kit ELISA

Sujet:

Protein Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC. .

Background: Vascular endothelial growth factor receptor 2(VEGFR-2) also known as Kinase insert domain receptor(KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.

Synonyms: Vascular endothelial growth factor receptor 2,VEGFR-2,2.7.10.1,Fetal liver kinase 1,FLK-1,Kinase insert domain receptor,KDR,Protein-tyrosine kinase receptor flk-1,CD309,KDR,FLK1, VEGFR2,

Full Gene Name: Vascular endothelial growth factor receptor 2

Cellular Localisation: Cell junction . Endoplasmic reticulum . Localized with RAP1A at cell-cell junctions (By similarity). Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610)..

ID gène: 3791

UniProt: P35968

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling