VEGFR2/CD309 Kit ELISA

N° du produit ABIN6719857

Détail du produit VEGFR2/CD309 Kit ELISA

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD309, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), Protein tyrosine kinase receptor Flk 1, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR 2, VEGFR2

Background: Vascular endothelial growth factor receptor 2(VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.

Gene Name: KDR

Production: Natural and recombinant mouse VEGFR2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A20-E762

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse VEGFR2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse VEGFR2 standard solution and each sample be measured in duplicate.

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Autre désignation: VEGFR2 - KDR (VEGFR2 Produits)

Synonymes: CD309 Kit ELISA, FLK1 Kit ELISA, VEGFR Kit ELISA, VEGFR2 Kit ELISA, 6130401C07 Kit ELISA, Flk-1 Kit ELISA, Flk1 Kit ELISA, Krd-1 Kit ELISA, Ly73 Kit ELISA, VEGFR-2 Kit ELISA, sVEGFR-2 Kit ELISA, Vegfr-2 Kit ELISA, FLK-1 Kit ELISA, flk-1 Kit ELISA, vegfr-2 Kit ELISA, KDR Kit ELISA, FLK/KDR Kit ELISA, flk1 Kit ELISA, flk1b Kit ELISA, kdrb Kit ELISA, si:busm1-205d10.1 Kit ELISA, si:ch211-254j6.1 Kit ELISA, si:ch211-278f21.4 Kit ELISA, wu:fc31a09 Kit ELISA, fk52c05 Kit ELISA, flk Kit ELISA, kdr Kit ELISA, kdra Kit ELISA, vegfr2 Kit ELISA, vegfr4 Kit ELISA, vegr2 Kit ELISA, wu:fk52c05 Kit ELISA, kinase insert domain receptor Kit ELISA, kinase insert domain protein receptor Kit ELISA, vascular endothelial growth factor receptor kdr-like Kit ELISA, kinase insert domain receptor (a type III receptor tyrosine kinase) Kit ELISA, kinase insert domain receptor like Kit ELISA, KDR Kit ELISA, Kdr Kit ELISA, LOC100136647 Kit ELISA, kdr Kit ELISA, kdrl Kit ELISA

ID gène: 16542

NCBI Accession: NP_034742

UniProt: P35918

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling