TACI Kit ELISA

N° du produit ABIN1889340

Détail du produit TACI Kit ELISA

Antigène: TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))

Épitope: AA 2-128

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.9-3000 pg/mL

Seuil minimal de détection: 46.9 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF13B/TACI

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A2-C128

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A2-C128

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse TNFRSF13B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF13B has been precoated onto 96-well plates. Standards(NSO, A2-C128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF13B amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL mouse TNFRSF13B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse TNFRSF13B standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 367, Standard deviation: 16.5, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 926, Standard deviation: 35.2, CV(%): 3.8
• Sample 3: n=16, Mean(pg/ml): 1624, Standard deviation: 86.1, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 241, Standard deviation: 13.5, CV(%): 5.6
• Sample 2: n=24, Mean(pg/ml): 1068, Standard deviation: 50.2, CV(%): 4.7
• Sample 3: n=24, Mean(pg/ml): 1867, Standard deviation: 113.9, CV(%): 6.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))

Autre désignation: TNFRSF13B (TNFRSF13B Produits)

Synonymes: CD267 Kit ELISA, CVID Kit ELISA, CVID2 Kit ELISA, RYZN Kit ELISA, TACI Kit ELISA, TNFRSF14B Kit ELISA, TNFRSF13B Kit ELISA, 1200009E08Rik Kit ELISA, Taci Kit ELISA, TNF receptor superfamily member 13B Kit ELISA, tumor necrosis factor receptor superfamily, member 13b Kit ELISA, TNFRSF13B Kit ELISA, Tnfrsf13b Kit ELISA

Sujet:

Protein Function: Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity (By similarity). .

Background: Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor(TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand(CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.

Synonyms: Tumor necrosis factor receptor superfamily member 13B,Transmembrane activator and CAML interactor,CD267,Tnfrsf13b,Taci,

Full Gene Name: Tumor necrosis factor receptor superfamily member 13B

Cellular Localisation: Membrane, Single-pass type III membrane protein.

ID gène: 57916

UniProt: Q9ET35