IL-1 beta Kit ELISA

N° du produit ABIN2042514

Ce kit ELISA Souris IL-1 beta est un kit ELISA Colorimetric conçu pour quantifier Souris IL-1 beta.

Aperçu rapide pour IL-1 beta Kit ELISA (ABIN2042514)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 12.5-800 pg/mL

Application: ELISA

Détail du produit IL-1 beta Kit ELISA

Seuil minimal de détection: 12.5 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients: 96-well plate pre-coated with anti-IL-1 beta, Mouse
Lyophilized recombinant IL-1 beta, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Mouse IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin-1 ( IL-1 ) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1 , a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1 and, to a lesser extent, IL-1 are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1 and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1 , which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin-1 beta (IL-1 Beta)

ID gène: 16176

UniProt: P10749

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome