CARTPT Kit ELISA

N° du produit ABIN2043177

Détail du produit CARTPT Kit ELISA

Antigène: CARTPT (CART Prepropeptide (CARTPT))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.055 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Cocaine And Amphetamine Regulated Transcript (CART) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cocaine And Amphetamine Regulated Transcript (CART), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cocaine And Amphetamine Regulated Transcript (CART) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CARTPT (CART Prepropeptide (CARTPT))

Autre désignation: Cocaine And Amphetamine Regulated Transcript (CART) (CARTPT Produits)

Synonymes: CART Kit ELISA, Cart Kit ELISA, CARTPT Kit ELISA, CART prepropeptide Kit ELISA, CARTPT Kit ELISA, Cartpt Kit ELISA

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour